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Journal / Immunology and Infection / 从富足人类记忆B细胞群中选择和恢复具有所需特异性的抗体的单细胞筛选方法…
JoVE Journal
Immunology and Infection

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从富足人类记忆B细胞群中选择和恢复具有所需特异性的抗体的单细胞筛选方法
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从富足人类记忆B细胞群中选择和恢复具有所需特异性的抗体的单细胞筛选方法

Article DOI: 10.3791/59809-v • 09:07 min • August 22nd, 2019
August 22nd, 2019 •
1, 1, 1, 2, 1
1World Without Disease Accelerator, Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson

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BSelex 方法用于识别和从人类外周血单核细胞中识别和回收单个抗原特异性抗体，将流式细胞学与单细胞 PCR 和克隆相结合。

Tags

免疫学和感染，第150期，B细胞表，B细胞受体，单细胞克隆，细胞分类，抗体发现，B细胞克隆，记忆B细胞，重链，轻链
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