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JoVE Journal
Biochemistry

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

使用自动工作站进行质谱测量的等离子体样品制备
 
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使用自动工作站进行质谱测量的等离子体样品制备

Article DOI: 10.3791/59842-v 07:12 min April 24th, 2020
April 24th, 2020

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在这里,我们提出了一种自动化血浆蛋白消化方法,用于基于质谱的定量蛋白质组分析。在此协议中,蛋白质变性、还原、烷基化和胰蛋白酶消化反应的液体转移和孵育步骤得到简化和自动化。准备一个96孔板需要大约5个小时,其精度要求。

Tags

生物化学, 问题 158, 液色谱- 串联质谱 (LC/MS/MS), 液色谱选择反应监测 (LC-SRM), 自动化, 蛋白质样品制备, 可重复性, 高通量
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