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Developmental Biology

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Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Article DOI: 10.3791/59965-v • 06:57 min • July 20th, 2019
July 20th, 2019 •
1, 1,2, 1,3, 1,4, 1, 1, 4, 3, 1
1MRC Centre for Regenerative Medicine, University of Edinburgh, 2UCL Great Ormond Street Institute of Child Health, University College London, 3Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences, Brunel University London, 4School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin

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This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application.

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Serum-free Production Three-dimensional Hepatospheres Pluripotent Stem Cells Methogenetics Fine Materials Cell Assembly Necrotic Centers Loss Of Phenotype Agarose Molds Low-melting Temperature Agarose Distilled Water Agarose Microplate 12-well Plate DPBS With Calcium And Magnesium Cell Suspension Undifferentiated Culture HPSCs Cell Dissociation Reagent
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