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Journal / Developmental Biology / Dissection, Culture and Analysis of Primary…
JoVE Journal
Developmental Biology

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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Article DOI: 10.3791/60051-v • 09:33 min • October 3rd, 2019
October 3rd, 2019 •
*1,2, *1,3, 1, 1, 1,3, 2,4, 3, 1
1Centre for Craniofacial and Regenerative Biology, King's College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research, University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics, King's College London, 4Department of Biological Applications and Technology, University of Ioannina
* These authors contributed equally

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Dieses Protokoll beschreibt die Zerlegung und Kultur von kranialen neuralen Kammzellen aus Mausmodellen, in erster Linie für die Untersuchung der Zellmigration. Wir beschreiben die verwendeten Live-Bildgebungstechniken und die Analyse von Geschwindigkeits- und Zellformänderungen.

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Entwicklungsbiologie Ausgabe 152 Zellkultur Live-Bildgebung Maus-Kranial-Neuralkamm Zellmigration epitheliaal-mesenchymaler Übergang Zellmotilität
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