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Verbesserte Lebensfähigkeit für Ex vivo 3D Hydrogelkulturen von patientenabgeleiteten Xenografts in einer perfused Microfluidic Platform
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Verbesserte Lebensfähigkeit für Ex vivo 3D Hydrogelkulturen von patientenabgeleiteten Xenografts in einer perfused Microfluidic Platform

Article DOI: 10.3791/60872-v • 12:12 min • December 5th, 2020
December 5th, 2020 •
1, 2, 3, 4, 1,5,6, 1,7, 2, 3, 1,5,6
1Department of BioSciences, Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering, Rice University, 6Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

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Dieses Protokoll zeigt Methoden, um eine erweiterte In-vitro-Kultur von patientenabgeleiteten Xenografts (PDX) zu ermöglichen. Ein Schritt verbessert die Gesamtlebensfähigkeit von mehrzelligen Clusterkulturen in 3D-Hydrogelen durch die einfache Entfernung nicht lebensfähiger Einzelzellen. Ein sekundärer Schritt veranschaulicht Best Practices für PDX-Kultur in einer durchsetzten mikrofluidischen Plattform.

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Krebsforschung Ausgabe 166 3D-Kultur Hydrogele Mikrofluidik von Patienten abgeleitete Xenografts Prostatakrebs In-vitro-Krebsmodelle Arzneimittel-Screening mit hohem Durchsatz Perfusion
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