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Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Article DOI: 10.3791/61138-v • 09:02 min • June 10th, 2020
June 10th, 2020 •
*1, *2, *3, 1, 1, 1, 2, 3
1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, 3Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio
* These authors contributed equally

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요약
Automatic Translation

CENP-A ubiquitylation is an important requirement for CENP-A deposition at the centromere, inherited through dimerization between cell division, and indispensable to cell viability. Here we describe mass spectrometry analysis to identify ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A) protein.

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Mass Spectrometry Analysis Identify Ubiquitylation EYFP-tagged CENP-A EYFP-CENP-A Human CENP-A Post-translational Modifications Protein A Beads Anti-GFP Antibody Buffer A1 Immunoprecipitation Reaction End-to-end Rotation Centrifuge Supernatant Lyse Cells Sonication Freeze/thaw Protein Concentrations
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