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Visualisering af DNA Reparation Proteiner Interaktion ved Immunofluorescence

Article DOI: 10.3791/61447-v • 07:55 min • June 26th, 2020
June 26th, 2020 •
1,2, 1,2
1Department of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio, 2Mays Cancer Center at UT Health San Antonio MD Anderson Cancer Center

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Efter DNA-skader aktiverer humane celler vigtige reparationsveje for at genoprette integriteten af deres genom. Her beskriver vi metoden til indirekte immunfluorescens som et middel til at detektere DNA-reparationsproteiner, analysere deres rumlige og tidsmæssige rekruttering og hjælpe med at afhøre proteinproteininteraktion på steder med DNA-skader.

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Biologi Immunofluorescence co-lokalisering nuklear foci bestråling-induceret foci DNA-skader reparation
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