Microscopy Imaging
Microscopy Imaging - Related Videos
11:41
Rapid Analysis and Exploration of Fluorescence Microscopy Images
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Cited by 3 •
2014
Here we describe a workflow for rapidly analyzing and exploring collections of fluorescence microscopy images using PhenoRipper, a recently developed image-analysis platform.
09:26
Experimental Screening Protocols, Immunocytochemistry and Microscopy-based Imaging Techniques for Penium margaritaceum
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2025
This report describes the fundamental methods used to culture and experimentally manipulate the unicellular streptophyte alga, Penium margaritaceum. It also provides fundamental protocols of microscopy-based imaging, including live cell labeling with monoclonal antibodies and other fluorescent probes and scanning electron microscopy.
14:11
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
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Cited by 10 •
2014
This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved detail.
10:48
Imaging Biological Samples with Optical and Confocal Microscopy
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Optical microscopes have been around for centuries, and while they reached their theoretical limitation of resolution decades ago, new equipment and techniques, such as confocal and digital image processing, have created new niches within the field of optical imaging. The best optical microscopes will typically have a resolution down to 200 nm in ideal...
10:03
Bacterial Immobilization for Imaging by Atomic Force Microscopy
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Cited by 36 •
2011
Live Gram-negative and Gram-positive bacteria can be immobilized on gelatin-coated mica and imaged in liquid using Atomic Force Microscopy (AFM).
FAQs
Microscopy imaging gains biological contrast by combining the signal collected from a specimen with a suitable labeling strategy. Fluorescent probes or antibodies can mark selected molecular or cellular features, while transmitted, reflected, or emitted signals provide the image-forming information. This combination helps distinguish relevant structures within complex infected tissues or cell preparations.
These signals provide different routes for collecting information from a specimen: transmission records signal passing through it, reflection records signal returned from it, and emission captures signal produced by the specimen or its labels. Recognizing this distinction clarifies how an image represents biological organization and helps researchers relate visible patterns to the observed sample.
Fluorescent probes and antibodies add molecular specificity to an image by marking selected targets rather than leaving all structures visually undifferentiated. Their signals can be interpreted alongside cellular shape and tissue organization, allowing investigators to connect molecular recognition with the location of cells, pathogens, or affected regions. This is especially useful when infection studies require spatial context.
Following cellular morphology over time can reveal changes that a single image would miss. In infection research, temporal imaging helps relate evolving cell shape and organization to pathogen localization, immune-cell interactions, or tissue damage. It therefore adds a dynamic dimension to observations and supports interpretation of host-pathogen behavior and treatment-related changes.
A microscopy imaging workflow begins by selecting the biological specimen and the signal to be captured, then directing light or electrons onto the sample. The resulting transmitted, reflected, or emitted signal is recorded as an image. Researchers may enhance contrast with fluorescent probes or antibodies before examining organization, localization, interactions, morphology, or damage.
It can show where pathogens are located, how immune cells interact with them, and whether tissues display structural damage. These observations preserve spatial relationships that are not conveyed by molecular recognition alone. In host-pathogen studies, the resulting images help connect the presence of infectious agents with cellular and tissue-level responses.
The method supports infection diagnosis, host-pathogen investigation, vaccine research, and evaluation of antimicrobial or immunomodulatory treatments. Its value extends from identifying pathogen-associated patterns to assessing immune-cell behavior, tissue condition, and changes in cellular morphology. Because it links molecular signals with spatial and temporal behavior, imaging can provide context for comparing biological states or treatment outcomes.