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A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Article DOI: 10.3791/61506-v • 10:39 min • September 17th, 2020
September 17th, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Biology, William Paterson University

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Here is a protocol to identify genetic interactions through an increased copy number suppressor screen in Saccharomyces cerevisiae. This method allows researchers to identify, clone, and test suppressors in short-lived yeast mutants. We test the effect of the copy number increase of SIR2 on lifespan in an autophagy null mutant.

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Suppressor Screen Genetic Links Chronological Lifespan Saccharomyces Cerevisiae Aging Cellular Processes Organismal Death Population Variability Genetic Factors Environmental Factors Diet Lifespan Extension Health Span Hallmarks Of Aging Mutations Telomeres Dysregulation Protein Homeostasis Respiration Problems Reactive Oxygen Species Model Organisms Budding Yeast Saccharomyces Cerevisiae
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