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Journal / Biology / Gewinnung primärer Osteozyten durch murine…
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Gewinnung primärer Osteozyten durch murine Schädelstockfraktionierung von GFP-exprimierenden Osteozyten
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Gewinnung primärer Osteozyten durch murine Schädelstockfraktionierung von GFP-exprimierenden Osteozyten

Article DOI: 10.3791/61513-v • 07:22 min • June 2nd, 2020
June 2nd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

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Dieses Protokoll beschreibt die Präparation von neonatalen dmp1-Topas-Mausschädeln und die Isolierung von Osteozyten, die das grün fluoreszierende Protein exprimieren, durch Zellverdau und Fraktionierung sowie die Osteozytenpräparation für die fluoreszenzaktivierte Zellsortierung (FACS).

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Retraktion Ausgabe 160 Primärer Osteozyten Dmp1 SOST GFP Fraktionierung FACS
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