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Journal / Biology / Sonder la cinétique de l’ARNm dans l’espace et le …
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Sonder la cinétique de l’ARNm dans l’espace et le temps à Escherichia coli à l’aide de l’hybridation de fluorescence in situ à deux couleurs
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Sonder la cinétique de l’ARNm dans l’espace et le temps à Escherichia coli à l’aide de l’hybridation de fluorescence in situ à deux couleurs

Article DOI: 10.3791/61520-v • 10:01 min • July 30th, 2020
July 30th, 2020 •
1,2,3,4, 1,2
1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

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Ce protocole décrit une application de l’hybridation in situ de fluorescence monomolécule (smFISH) pour mesurer la cinétique in vivo de la synthèse et de la dégradation de l’ARNm.

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Biologie Numéro 161 smFISH imagerie d’ARN localisation d’ARNm cellule unique molécule unique transcription élongation de transcription terminaison prématurée dégradation de l’ARNm
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