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Journal / Immunology and Infection / عزل الماوس الكلى المقيم CD8+ الخلايا T لتحليل…
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Immunology and Infection

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JoVE Journal Immunology and Infection
عزل الماوس الكلى المقيم CD8+ الخلايا T لتحليل تدفق cytometry
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عزل الماوس الكلى المقيم CD8+ الخلايا T لتحليل تدفق cytometry

Article DOI: 10.3791/61559-v • 06:07 min • June 27th, 2020
June 27th, 2020 •
1,2, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University

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بعد العدوى الفيروسية, الكلى المرافئ عددا كبيرا نسبيا من CD8+ الخلايا T ويوفر فرصة لدراسة غير المخاطية Tالخلايا RM. هنا، نحن وصف بروتوكول لعزل الخلايا الليمفاوية الكلى الماوس لتحليل تدفق الخلايا.

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هذا الشهر في JoVE ، العدد 160 ، CD8 ، مقيم في الكلى ، LCMV ، عملية استئصال الخلايا المتدفقة ، الماوس ، وضع العلامات داخل الأوعية
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