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Immunology and Infection

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Isolement des cellules CD8+ T résidentes du rein de souris pour l’analyse de cytométrie de flux
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Isolement des cellules CD8+ T résidentes du rein de souris pour l’analyse de cytométrie de flux

Article DOI: 10.3791/61559-v • 06:07 min • June 27th, 2020
June 27th, 2020 •
1,2, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University

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Suite à une infection virale, le rein abrite un nombre relativement important de cellules CD8+ T et offre la possibilité d’étudier les lymphocytes TRM non muqueux. Ici, nous décrivons un protocole pour isoler les lymphocytes rénaux de souris pour l’analyse de cytométrie de flux.

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Ce mois-ci dans JoVE Numéro 160 CD8 résident rénal LCMV cytométrie de flux souris étiquetage intravasculaire
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