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Udforskning af fedtvævsstruktur ved methylsalicylatclearing og 3D-billeddannelse
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Udforskning af fedtvævsstruktur ved methylsalicylatclearing og 3D-billeddannelse

Article DOI: 10.3791/61640-v • 10:10 min • August 19th, 2020
August 19th, 2020 •
1, 1, 2, 3, 1, 4, 4, 1, 1
1Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France

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Her beskriver vi en enkel, billig og hurtig clearing metode til at løse 3D-strukturen af både mus og humanhvidt fedtvæv ved hjælp af en kombination af markører til at visualisere vaskulatur, kerner, immunceller, neuroner, og lipid-dråbe pels proteiner ved fluorescerende billeddannelse.

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Biologi Fedtvæv clearing lys mikroskopi 3-D hele væv fedme morfologi
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