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Embryo's van Culex-muggen voorbereiden en injecteren om null-mutaties te genereren met CRISPR/Cas9

Article DOI: 10.3791/61651-v • 07:45 min • September 10th, 2020
September 10th, 2020 •
1, 2
1Department of Entomology, The Ohio State University, 2Insect Transformation Facility, Institute for Bioscience and Biotechnology Research, University of Maryland

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CRISPR/Cas9 wordt steeds vaker gebruikt om genfunctie in niet-modelorganismen te karakteriseren. Dit protocol beschrijft hoe knock-out lijnen van Culex pipienste genereren, van het bereiden van injectiemengsels tot het verkrijgen en injecteren van muggenembryo's, evenals hoe geïnjecteerde muggen en hun nakomelingen op te wekken, te kruisen en te screenen op gewenste mutaties.

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Biologie Designing guide RNAs micromanipulatie micro-injection mosquito embryo mutatie screening Northern house mosquito genome editing
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