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Journal / Genetics / In Vivo CRISPR/Cas9 Screening para evaluar…
JoVE Journal
Genetics

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JoVE Journal Genetics
In Vivo CRISPR/Cas9 Screening para evaluar simultáneamente la función génica en la piel del ratón y la cavidad oral
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In Vivo CRISPR/Cas9 Screening para evaluar simultáneamente la función génica en la piel del ratón y la cavidad oral

Article DOI: 10.3791/61693-v • 07:52 min • November 2nd, 2020
November 2nd, 2020 •
1, 1,2, 1,2, 3, 1,2
1Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics, University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

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Aquí describimos una metodología de detección CRISPR/Cas9 rápida y directa in vivo utilizando inyecciones lentivirales embrionarias de tero guiadas por ultrasonido para evaluar simultáneamente las funciones de varios genes en la piel y la cavidad oral de ratones inmunocompetentntes.

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Este mes en JoVE Número 165 CRISPR in vivo micro inyección guiada por ultrasonido cola larga biblioteca CRISPR modelos de ratón de cáncer pantalla in vivo rápida
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