샌드 플라이 (플레보토무스 파파타시) CRISPR/Cas9 돌연변이 발생을 위한 배아 미세 주입
7,150 Views
•
05:44 min
•
November 17, 2020
DOI:
Summary
Automatically generated
이 프로토콜은 태아 수집, 주사, 곤충 사육 및 식별뿐만 아니라 관심있는 돌연변이의 선택 : 모래 파리에 CRISPR / Cas9 표적 돌연변이 발생의 단계를 자세히 설명합니다.
Related Videos
09:45
Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
Related Videos
8974 Views
09:05
Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation
Related Videos
12255 Views
05:59
Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus
Related Videos
5487 Views
08:30
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator
Related Videos
2561 Views
05:32
Embryo Microinjection for Transgenesis in Drosophila
Related Videos
1845 Views
06:47
Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9
Related Videos
770 Views
06:37
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)
Related Videos
4402 Views
06:08
Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica
Related Videos
4338 Views
05:44
Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis
Related Videos
7.1K Views
08:19
Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
Related Videos
2.3K Views
Read Article
Cite this Article
Louradour, I., Ghosh, K., Inbar, E., Sacks, D. L., Aluvihare, C., Harrell II, R. A. Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis. J. Vis. Exp. (165), e61924, doi:10.3791/61924 (2020).
Copy