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Journal / Genetics / CRISPR/Cas9 Edición del gen C. elegans rbm-3.2…
JoVE Journal
Genetics

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JoVE Journal Genetics
CRISPR/Cas9 Edición del gen C. elegans rbm-3.2 utilizando el marcador de detección co-CRISPR dpy-10 y complejos de ribonucleoproteína ensamblados.
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CRISPR/Cas9 Edición del gen C. elegans rbm-3.2 utilizando el marcador de detección co-CRISPR dpy-10 y complejos de ribonucleoproteína ensamblados.

Article DOI: 10.3791/62001-v • 07:46 min • December 11th, 2020
December 11th, 2020 •
1, *1, *1, *1, 1
1Department of Chemistry and Biochemistry, The University of Tulsa
* These authors contributed equally

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El objetivo de este protocolo es permitir la edición CRISPR/Cas9 sin fisuras del genoma de C. elegans utilizando complejos de ribonucleoproteína ensamblados y el marcador co-CRISPR dpy-10 para el cribado. Este protocolo se puede utilizar para realizar una variedad de modificaciones genéticas en C. elegans, incluidas inserciones, deleciones, reemplazos de genes y sustituciones de codones.

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Genética Número 166 CRISPR/Cas9 C. elegans complejosde ribonucleoproteínas ingeniería genómica rbm-3.2,edición de genes microinyección cribado CRISPR
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