3,424 Views
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07:26 min
May 26, 2021
DOI:
10.3791/62155-v
이 프로토콜은 제브라피시의 토토 라벨링 및 다차원 이미징의 접근 방식을 초기 눈 발달에 설명합니다. 당사는 레이저 스캐닝 공초점 현미경을 이용한 라벨링, 임베딩 및 4차원(4D) 이미징을 설명하고, 광학컵 형태발생의 해부 메커니즘을 위한 데이터 집합의 획득을 최적화하기 위한 고려 사항을 설명합니다.
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Cite this Article
Lusk, S., Casey, M. A., Kwan, K. M. 4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis. J. Vis. Exp. (171), e62155, doi:10.3791/62155 (2021).
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