JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Cell Biology,University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine, 3Department of Cardiology,University of Virginia School of Medicine, 4Hematovascular Biology Center,University of Virginia School of Medicine, 5Yale Cardiovascular Research Center,Yale University School of Medicine
Chapters
- 00:04Introduction
- 00:38Obtain Mouse Eyes for Retinal Isolation and Isolate Mouse Retinal Tissue
- 02:30Digest Retinal Tissue into a Single Cell Suspension and Immunostain the Cells with Antibodies
- 04:07Prepare for Fluorescence‐Activated Cell Sorting
- 05:19Isolate Viable Endothelial Cells via FACS
- 07:18Results: Analysis of Isolated Endothelial Cells and Next‐Generation Sequencing After Isolation
- 09:03Conclusion
