Intravital Subcellular Microscopy of the Mammary Gland(유선의 생체 내 세포내 현미경)

Overview

This protocol presents a technique for intravital imaging of the lactating mouse mammary gland utilizing laser scanning confocal and multiphoton microscopy. The study emphasizes the importance of maintaining optical resolution while minimizing blood loss during surgery.

Key Study Components

Research Area

• Microscopy
• Imaging techniques

Background

• Intravital imaging provides insights into gland morphology and cellular dynamics.
• Understanding the lactating mammary gland is crucial for developmental biology and cell biology.

Methods Used

• Laser scanning confocal and multiphoton microscopy
• Lactating mouse mammary gland
• Use of EGFP and BODIPY dyes

Main Results

• Distinct labeling of secretory epithelial cells and lipid droplets.
• Time-lapse analysis showed dynamic movement of lipid droplets.
• Comprehensive survey of gland morphology using dual fluorescence.

Conclusions

• The study demonstrates a method to visualize complex biological processes in real-time.
• This approach is relevant for further understanding mammary gland physiology and disease.

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