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Enkeltcelle proteomisk forberedelse til massespektrometrianalyse ved hjælp af frysevarmelysis og en isobarisk bærer

Article DOI: 10.3791/63802-v • 06:13 min • December 9th, 2022
December 9th, 2022 •
*1, 1,2,3, *1
1Department of Bioengineering, Northeastern University, 2Barnett Institute, Northeastern University, 3Department of Biology, Northeastern University
* These authors contributed equally

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I denne protokol beskriver vi, hvordan man forbereder pattedyrceller til enkeltcelleproteomanalyse via massespektrometri ved hjælp af kommercielt tilgængelige reagenser og udstyr med muligheder for både manuel og automatisk pipettering.

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Biologi udgave 190
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