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Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

Article DOI: 10.3791/63972-v • 07:49 min • June 2nd, 2022
June 2nd, 2022 •
*1,2, *2, 1,2
1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Physiology, College of Medicine, National Cheng Kung University
* These authors contributed equally

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요약
Automatic Translation

Gut-derived microbial metabolites have multifaceted effects leading to complex behavior in animals. We aim to provide a step-by-step method to delineate the effects of gut-derived microbial metabolites in the brain via intracerebroventricular delivery via a guide cannula.

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Intracerebroventricular Delivery Gut-derived Microbial Metabolites Freely Moving Mice Gut Microbiota Mouse Brain Behavior Precise Method Meticulous Method Validated Method Gut Metabolites Brain Research Stereotaxic Frame Incisor Bar Nose Cone Mask Nociception Surgical Site Fur Trimming Analgesic Ketoprofen Eye Ointment Head Fixation Chlorhexidine Swab Disinfection Scalp Incision Skull Wiping Stereotaxic Coordinates
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