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JoVE Journal
Bioengineering

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Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System
 

Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System

Article DOI: 10.3791/64281-v 09:56 min December 23rd, 2022
December 23rd, 2022

챕터

요약

The present protocol describes the design, fabrication, and characterization of a microfluidic system capable of aligning, immobilizing, and precisely compressing hundreds of Drosophila melanogaster embryos with minimal user intervention. This system enables high-resolution imaging and recovery of samples for post-stimulation analysis and can be scaled to accommodate other multicellular biological systems.

Tags

Microfluidics Compression System Multicellular Organisms Mechanostimulation Fabricate Microfabrication Techniques Silicon Wafer Hexamethyldisiloxane SU-8 2100 Photoresist Viscosity Dehydration Bake Mechanical Stress Visualizing Samples Immobilization Alignment Live Imaging
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