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Bioengineering

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JoVE Journal Bioengineering
Digitala genkretsar baserade på CRISPR-Cas-system och anti-CRISPR-proteiner
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Digitala genkretsar baserade på CRISPR-Cas-system och anti-CRISPR-proteiner

Article DOI: 10.3791/64539-v • 10:46 min • October 18th, 2022
October 18th, 2022 •
*1, *1, 1
1School of Pharmaceutical Science and Technology, Tianjin University
* These authors contributed equally

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CRISPR-Cas-system och anti-CRISPR-proteiner integrerades i schemat av booleska grindar i Saccharomyces cerevisiae. De nya små logiska kretsarna visade god prestanda och fördjupade förståelsen för både dCas9/dCas12a-baserade transkriptionsfaktorer och egenskaperna hos anti-CRISPR-proteiner.

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Retraktion utgåva 188 CRISPR-Cas-system dSpCas9 denAsCas12a dLbCas12a typ II-A anti-CRISPR-proteiner typ V-A anti-CRISPR-proteiner digitala genkretsar Saccharomyces cerevisiae syntetisk biologi
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