Name: 난소 절제술 쥐에서 Bazi Bushen 캡슐의 에스트로겐 유사 효과 (동영상) | JoVE Journal
Uploaded: 2023-04-07T14:40:00.000+00:00
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Description: 배우세요 난소 절제술 쥐 모델에서 Bazi Bushen 캡슐이 RANKL/OPG 신호경로를 조절하여 에스트로겐 유사 효과를 나타내는 메커니즘을 micro-CT와 면역조직화학을 통해 분석하는 방법을.

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난소 절제술 쥐에서 bazi bushen 캡슐의 에스트로겐 유사 효과

Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats

경골의 뼈 미세구조는 마이크로 CT 스캔으로 평가되었습니다 난소 절제술을 받은 쥐를 BZBS로 처리하면 OVX에 의한 섬유주 구조 변화가 유의하게 감소했습니다. 복원된 우측 경골의 micro-CT 이미지(그림 1A,B)에서 볼 수 있듯이 OVX 그룹의 섬유주골은 SHAM 그룹에 비해 BMD(그림 1C), BV/TV(그림 1D), Tb.Th(그림 1E), Tb.N(그림 1F) 및 Tb.Sp(그림 1G)가 유의하게 감소한 것으로 나타났습니다. OVX 투여군과 비교했을 때, BZBS 치료군에서 우경골의 BV/TV, Tb.N 및 Tb.Th.는 유의하게 증...

난소 절제술 쥐에서 Bazi Bushen 캡슐의 에스트로겐 유사 효과

This study aims to show the estrogen-like effect of Bazi Bushen capsule (BZBS), a Chinese herbal compound, in ovariectomized mice. Female Sprague-Dawley ...

estrogen-like-effect-of-bazi-bushen-capsule-in-ovariectomized-rats

Research

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의학

silastic 캡슐과 peroral Nutella 의한 Ovariectomy 및 17β-estradiol의 후속 교체는 쥐 및 생쥐에서 증명하고 있습니다.

고양이와 쥐의 Ovariectomy 및 17β-estradiol 대체 : 비주얼 데모

에스트로겐과 같은 생식선 호르몬은 공포, 소멸, 기억을 포함한 여러 실험 패러다임에서 기억 형성을 조절합니다. 이 프로토콜은 발정 주기 모니터링 및 외인성 에스트로겐 투여를 포함하여 자연적으로 순환하는 암컷에서 사멸 중 특히 생식선 호르몬의 영향을 조사하기 위한 일련의 방법을 설명합니다.

당연히 여성 쥐 사이클링에 에어컨 공포의 멸종 유학을위한 프로토콜

임상적으로, 갱년기 여성의 에스트로겐 결핍은 지질 장애및 동맥 경화증의 발생률을 악화시킬 수 있습니다. 우리는 apoE-/- 마우스에 있는 이중 등측 절개를 통해 양측 난소 절제술에 의하여 생체 내 에스트로겐 부족 모형을 설치했습니다. 마우스 모델은 폐경 후 심혈관 기능 장애의 외인성 에스트로겐 치료를 스크리닝하는 데 적용가능하다.

폐경 후 심장 혈관 기능 장애의 외인성 에스트로겐 치료를 선별하기위한 생체 내 에스트로겐 결핍 마우스 모델

다양한 성선외 조직과 장기의 난포 자극 호르몬(FSH)은 여러 질병의 발병과 관련이 있습니다. 난소 절제술 및 난포자극호르몬 처리 마우스 모델(OVF)을 사용하여 난포자극호르몬의 성선외 작용을 탐색할 수 있습니다.

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model (생쥐 모델에서 모낭 자극 호르몬의 생체 내 독립 효과 탐색)

이 프로토콜은 미니 펌프를 사용하여 제어된 레트로졸 방출을 통해 마우스에서 다낭성 난소 증후군(PCOS) 모델을 유도하는 기술을 소개합니다. 적절한 마취 하에 미니 펌프를 피하 주사하고, 미니 펌프 방출 일정 기간 후에 마우스에서 PCOS를 성공적으로 유도하였다.

생쥐에서 다낭성 난소 증후군을 유도하기 위한 수정된 기술

이 프로토콜은 테스토스테론 또는 에스트라디올과 시프로테론 아세테이트(트랜스젠더를 위한 인간 치료에 사용됨)의 피하 투여를 통해 성별을 확인하는 호르몬 요법을 모방한 두 가지 설치류 모델의 개발, 용량 설정, 관련 바이오마커 식별 및 효과 평가를 설명합니다.

성별 확인 호르몬 요법을 모방한 쥐 모델 수립

급성 심근 경색 (AMI)의 쥐 모델은 심장 pathophysiological 및 생리 기능에 MI의 결과를 연구하는 데 유용합니다.

고양이에서 급성 심근 경색

인간 pluripotent 줄기 세포 (hPSCs)은 다양한 질병을 치료하기 위해 무수히 많은 가능성이 있습니다. 이러한 세포의 유틸리티들은 신체의 모든 세포 유형으로 구별 수있다는 사실에있다. 여기 hPSCs의 pluripotence을 설명하는 데 사용됩니다 teratoma 분석을 설명합니다.

Testis 캡슐의 Teratoma 생성

작은 창자 이식은 되돌릴 장 실패 환자에 대한 인정 치료 옵션이되었습니다. 쥐의 orthotopic 작은 창자 이식 우리의 실험 모델은 장 이식을 복잡하게 기본 immunologic과 염증 프로세스를 처리 할 수​​있는 신뢰할 수있는 도구로 제공하고 있습니다.

쥐 Orthotopic 작은 창자 이식

우리는 시궁창에서 orthotopic 간 이식을위한 클래식 두 커프스 기술의 쉬운 설정 개정을 제시.

쥐에게 Orthotopic 간 이식

배우세요 난소 절제술 쥐 모델에서 Bazi Bushen 캡슐이 RANKL/OPG 신호경로를 조절하여 에스트로겐 유사 효과를 나타내는 메커니즘을 micro-CT와 면역조직화학을 통해 분석하는 방법을.

난소 절제술 쥐에서 Bazi Bushen 캡슐의 에스트로겐 유사 효과

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