A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models

Genri Numata; Eiki Takimoto

doi:10.3791/65384

쥐 심부전 모델에서 심박수 의존적 이완기 기능 평가를 위한 속도 제어 절차

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Biology

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A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models

쥐 심부전 모델에서 심박수 의존적 이완기 기능 평가를 위한 속도 제어 절차

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07:49 min

July 21, 2023

DOI: 10.3791/65384-v

Genri Numata^1,2, Eiki Takimoto^1,3

¹Department of Cardiovascular Medicine,The University of Tokyo Hospital, ²Department of Therapeutic Strategy for Heart Failure, Graduate School of Medicine,The University of Tokyo, ³Division of Cardiology, Department of Medicine,The Johns Hopkins Medical Institutions

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Overview

This study presents a protocol for assessing the pressure-volume relationship through transesophageal pacing in mouse models of heart failure, particularly focusing on the diastolic function. The protocol is designed to detect subtle changes in heart performance related to heart rate.

Key Study Components

Research Area

Cardiac function assessment
Heart failure models
Diastolic dysfunction evaluation

Background

Heart rate influence on cardiac characteristics
Limitations of treadmill exercise testing
Need for improved assessment methods for heart function

Methods Used

Atrial pacing protocol
Mouse models of heart failure
Transesophageal pacing technology

Main Results

Successfully identified cardiac dysfunction linked to heart rate changes
Demonstrated subtle performance changes in heart function
Validated the approach for studying diastolic dysfunction

Conclusions

The protocol effectively evaluates diastolic function in heart failure models
Offers a novel approach to studying heart rate-dependent cardiac functions

Frequently Asked Questions

What is the main objective of this study?

To develop a protocol that assesses the pressure-volume relationship in heart failure models.

How does this protocol improve upon traditional methods?

It addresses the limitations of treadmill testing by providing a more precise assessment of heart rate-dependent cardiac function.

What model system is used in this research?

Mouse models of heart failure are utilized for the experiments.

What does transesophageal pacing involve?

It is a technique used to assess heart function through pacing the heart via the esophagus.

Why is heart rate important in this study?

Heart rate changes are critical for understanding cardiac dysfunction, particularly in HFpEF.

What are the implications of this research?

This study offers new insights into the evaluation of diastolic dysfunction in heart failure, which can inform future studies.

How might this research impact future cardiac studies?

By providing a reliable method for assessing heart function, it may lead to better understanding and treatment of heart failure.

본 프로토콜은 심부전의 마우스 모델에서 이완기 기능을 평가하는 데 유용한 도구 역할을 하는 경식도 페이싱을 통해 압력-부피 관계를 얻는 방법을 설명합니다.

심박수 관련 관계는 특히 HFpEF에서 심장 특성을 결정하는 데 중요합니다. 이 프로토콜은 심박수에 따른 심장 기능을 평가하는 것을 목표로 합니다. 심박수 관련 심장 기능을 평가하는 데 사용할 수 있는 모든 실험 모델은 트레드밀 운동 테스트로 제한됩니다.

그러나 트레드밀 테스트는 체중과 같은 변수의 영향을 받습니다. 이러한 한계를 극복하기 위해 심방 페이싱 프로토콜을 중심으로 심박수와 관련된 이후 성능의 미묘하지만 중요한 변화를 감지했습니다. 당사의 프로토콜은 심박수 변화로 나타나는 심장 기능 장애를 식별하기 위한 다양한 도구를 제공합니다.

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