Name: Fusarium oxysporum f. sp. cubense Tropical Race 4를 검출하기 위한 복합 재조합효소 중합효소 증폭 CRISPR/Cas12a 분석 (동영상) | JoVE Journal
Uploaded: 2025-11-14T13:35:00.000+00:00
Duration: 6 min 47 s
Description: 학습하세요 Fusarium oxysporum f. sp. cubense Tropical Race 4를 검출하는 CRISPR/Cas12a 분석법을 배워보세요. 재조합효소 중합효소 증폭과 결합하여 신속하고 경제적인 현장 진단이 가능합니다.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Viljoen, A., Ma, L. J., Molina, A. B. Fusarium Wilt (Panama Disease) and Monoculture in Banana Production: Resurgence of a Century-Old Disease. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aViljoen%2C+A+%22Emerging+Plant+Diseases+and+Global+Food+Security%22">Emerging Plant Diseases and Global Food Security</a>. , The American Phytopathological Society. St. Paul, MN. (2020).</li>
<li>Viljoen, A., et al. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aViljoen%2C+A+%22Banana+Diseases+and+Pests%3A+Field+Guide+for+Diagnostics+and+Data+Collection%22">Banana Diseases and Pests: Field Guide for Diagnostics and Data Collection</a>. , IITA, Ibadan. (2017).</li>
<li>Dita, M. A., Waalwijk, C., Buddenhagen, I. W., Souza, M. T., Kema, G. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+molecular+diagnostic+for+tropical+race+4+of+the+banana%5BTitle%5D)+AND+%22Plant+Pathol%22%5BJournal%5D)">A molecular diagnostic for tropical race 4 of the banana.</a> Plant Pathol. 59 (2), 348-357 (2010).</li>
<li>Li, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Identification+and+application+of+a+unique+genetic+locus+in+diagnosis+of+Fusarium+oxysporum+f.+sp.+cubense+tropical+race+4%5BTitle%5D)+AND+%22Can+J+Plant+Pathol%22%5BJournal%5D)">Identification and application of a unique genetic locus in diagnosis of Fusarium oxysporum f. sp. cubense tropical race 4.</a> Can J Plant Pathol. 35 (4), 482-493 (2013).</li>
<li>Aguayo, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+a+hydrolysis+probe-based+real-time+assay+for+the+detection+of+tropical+strains+of+Fusarium+oxysporum+f.+sp.+cubense+race+4%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Development of a hydrolysis probe-based real-time assay for the detection of tropical strains of Fusarium oxysporum f. sp. cubense race 4.</a> PLoS One. 12 (2), e0171767(2017).</li>
<li>Matthews, M. C., Mostert, D., Ndayihanzamaso, P., Rose, L. J., Viljoen, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+detection+of+economically+important+Fusarium+oxysporum+f.+sp.+cubense+strains+in+Africa+in+plants%2C+soil+and+water%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Quantitative detection of economically important Fusarium oxysporum f. sp. cubense strains in Africa in plants, soil and water.</a> PLoS One. 15 (7), e0236110(2020).</li>
<li>Ordóñez, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+loop-mediated+isothermal+amplification+%28LAMP%29+assay+based+on+unique+markers+derived+from+genotyping+by+sequencing+data+for+rapid+in+planta+diagnosis+of+Panama+disease+caused+by+tropical+race+4+in+banana%5BTitle%5D)+AND+%22Plant+Pathol%22%5BJournal%5D)">A loop-mediated isothermal amplification (LAMP) assay based on unique markers derived from genotyping by sequencing data for rapid in planta diagnosis of Panama disease caused by tropical race 4 in banana.</a> Plant Pathol. 68 (9), 1682-1693 (2019).</li>
<li>Martzy, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Challenges+and+perspectives+in+the+application+of+isothermal+DNA+amplification+methods+for+food+and+water+analysis%5BTitle%5D)+AND+%22Anal+Bioanal+Chem%22%5BJournal%5D)">Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis.</a> Anal Bioanal Chem. 411, 1695-1702 (2019).</li>
<li>Ghouneimy, A., Mahas, A., Marsic, T., Aman, R., Mahfouz, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CRISPR-based+diagnostics%3A+Challenges+and+potential+solutions+toward+point-of-care+applications%5BTitle%5D)+AND+%22ACS+Synth+Biol%22%5BJournal%5D)">CRISPR-based diagnostics: Challenges and potential solutions toward point-of-care applications.</a> ACS Synth Biol. 12 (1), 1-16 (2023).</li>
<li>Chen, J. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CRISPR-Cas12a+target+binding+unleashes+indiscriminate+single-stranded+DNase+activity%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity.</a> Science. 360 (6387), 436-439 (2018).</li>
<li>Gootenberg, J. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nucleic+acid+detection+with+CRISPR-Cas13a%2FC2c2%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">Nucleic acid detection with CRISPR-Cas13a/C2c2.</a> Science. 356 (6336), 438-442 (2017).</li>
<li>Chen, Y., Mei, Y., Jiang, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Universal+and+high-fidelity+DNA+single+nucleotide+polymorphism+detection+based+on+a+CRISPR%2FCas12a+biochip%5BTitle%5D)+AND+%22Chem+Sci%22%5BJournal%5D)">Universal and high-fidelity DNA single nucleotide polymorphism detection based on a CRISPR/Cas12a biochip.</a> Chem Sci. 12 (12), 4455-4462 (2021).</li>
<li>Shin, K., Kwon, S. H., Lee, S. C., Moon, Y. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sensitive+and+rapid+detection+of+citrus+scab+using+an+RPA-CRISPR%2FCas12a+system+combined+with+a+lateral+flow+assay%5BTitle%5D)+AND+%22Plants+%28Basel%29%22%5BJournal%5D)">Sensitive and rapid detection of citrus scab using an RPA-CRISPR/Cas12a system combined with a lateral flow assay.</a> Plants (Basel). 10 (10), 2132(2021).</li>
<li>Zhang, Y. M., Zhang, Y., Xie, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+CRISPR%2FCas12a-based+DNA+detection+for+fast+pathogen+diagnosis+and+GMO+test+in+rice%5BTitle%5D)+AND+%22Mol+Breed%22%5BJournal%5D)">Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice.</a> Mol Breed. 40 (1), 11(2020).</li>
<li>Wang, Q., Qin, M., Coleman, J. J., Shang, W., Hu, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+and+sensitive+detection+of+Verticillium+dahliae+from+complex+samples+using+CRISPR%2FCas12a+technology+combined+with+RPA%5BTitle%5D)+AND+%22Plant+Dis%22%5BJournal%5D)">Rapid and sensitive detection of Verticillium dahliae from complex samples using CRISPR/Cas12a technology combined with RPA.</a> Plant Dis. 107 (6), 1664-1669 (2023).</li>
<li>Sun, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+and+sensitive+detection+of+two+fungal+pathogens+in+soybeans+using+the+recombinase+polymerase+amplification%2FCRISPR-Cas12a+method+for+potential+on-site+disease+diagnosis%5BTitle%5D)+AND+%22Pest+Manag+Sci%22%5BJournal%5D)">Rapid and sensitive detection of two fungal pathogens in soybeans using the recombinase polymerase amplification/CRISPR-Cas12a method for potential on-site disease diagnosis.</a> Pest Manag Sci. 80 (3), 1168-1181 (2024).</li>
<li>Chen, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+frontline%2C+rapid%2C+nucleic+acid-based+Fusarium+circinatum+detection+system+using+CRISPR%2FCas12a+combined+with+recombinase+polymerase+amplification%5BTitle%5D)+AND+%22Plant+Dis%22%5BJournal%5D)">A frontline, rapid, nucleic acid-based Fusarium circinatum detection system using CRISPR/Cas12a combined with recombinase polymerase amplification.</a> Plant Dis. 107 (6), 1902-1910 (2023).</li>
<li>Mu, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CRISPR-Cas12a-based+diagnostics+of+wheat+fungal+diseases%5BTitle%5D)+AND+%22J+Agric+Food+Chem%22%5BJournal%5D)">CRISPR-Cas12a-based diagnostics of wheat fungal diseases.</a> J Agric Food Chem. 70 (23), 7240-7247 (2022).</li>
<li>Li, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Visual+detection+of+Fusarium+temperatum+by+using+CRISPR-Cas12a+empowered+LAMP+assay+coupled+with+AuNPs-based+colorimetric+reaction%5BTitle%5D)+AND+%22LWT%22%5BJournal%5D)">Visual detection of Fusarium temperatum by using CRISPR-Cas12a empowered LAMP assay coupled with AuNPs-based colorimetric reaction.</a> LWT. 185, 115190(2023).</li>
<li>Liang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+an+RPA-based+CRISPR%2FCas12a+assay+in+combination+with+a+lateral+flow+strip+for+rapid+detection+of+toxigenic+Fusarium+verticillioides+in+maize%5BTitle%5D)+AND+%22Food+Control%22%5BJournal%5D)">Development of an RPA-based CRISPR/Cas12a assay in combination with a lateral flow strip for rapid detection of toxigenic Fusarium verticillioides in maize.</a> Food Control. 157, 110172(2024).</li>
<li>Matthews, M. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+combined+recombinase+polymerase+amplification+CRISPR%2FCas12a+assay+for+detection+of+Fusarium+oxysporum+f.+sp.+cubense+tropical+race+4%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">A combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4.</a> Sci Rep. 15 (1), 2436(2025).</li>
<li><a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22RNA+Clean+%26+Concentrator-5%3A+Instruction+Manual+Version+3.1.3%22">RNA Clean & Concentrator-5: Instruction Manual Version 3.1.3</a>. , Zymo Research Corporation. Irvine, CA. (2023).</li>
<li><a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Genie+III+user+manual%2C+instrument+software+version+v3.18.2%22">Genie III user manual, instrument software version v3.18.2</a>. , Optigene. Horsham, U.K. (2021).</li>
<li>Li, S. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CRISPR-Cas12a-assisted+nucleic+acid+detection%5BTitle%5D)+AND+%22Cell+Discov%22%5BJournal%5D)">CRISPR-Cas12a-assisted nucleic acid detection.</a> Cell Discov. 4, 1-4 (2018).</li>
<li>Magdama, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparative+analysis+uncovers+the+limitations+of+current+molecular+detection+methods+for+Fusarium+oxysporum+f.+sp.+cubense+race+4+strains%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Comparative analysis uncovers the limitations of current molecular detection methods for Fusarium oxysporum f. sp. cubense race 4 strains.</a> PLoS One. 14 (9), e0222727(2019).</li>
<li>Mckillop, C. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aMckillop%2C+C+%22Independent+review+blames+unreliable+test%2C+not+Biosecurity+Queensland%2C+for+false+Panama+diagnosis%22">Independent review blames unreliable test, not Biosecurity Queensland, for false Panama diagnosis</a>. , ABC News, Australia. (2016).</li>
<li>Mckillop, C., Craig, Z. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aMckillop%2C+C+%22Quarantine+lifted+on+Mareeba+banana+farm+suspected+of+having+Panama+disease%22">Quarantine lifted on Mareeba banana farm suspected of having Panama disease</a>. , ABC News, Australia. (2015).</li>
<li>Wang, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cas12aVDet%3A+A+CRISPR%2FCas12a-based+platform+for+rapid+and+visual+nucleic+acid+detection%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">Cas12aVDet: A CRISPR/Cas12a-based platform for rapid and visual nucleic acid detection.</a> Anal Chem. 91 (19), 12156-12161 (2019).</li>
<li>Fozouni, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Amplification-free+detection+of+SARS-CoV-2+with+CRISPR-Cas13a+and+mobile+phone+microscopy%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy.</a> Cell. 184 (2), 323-333 (2021).</li>
<li>Ding, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ultrasensitive+and+visual+detection+of+SARS-CoV-2+using+all-in-one+dual+CRISPR-Cas12a+assay%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay.</a> Nat Commun. 11 (1), 4711(2020).</li>
<li>Parida, M., Sannarangaiah, S., Dash, P. K., Rao, P. V., Morita, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Loop-mediated+isothermal+amplification+%28LAMP%29%3A+A+new+generation+of+innovative+gene+amplification+technique%3B+perspectives+in+clinical+diagnosis+of+infectious+diseases%5BTitle%5D)+AND+%22Rev+Med+Virol%22%5BJournal%5D)">Loop-mediated isothermal amplification (LAMP): A new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases.</a> Rev Med Virol. 18 (6), 407-421 (2008).</li>
<li>Zeng, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CRISPR-Cas12a-driven+MXene-PEDOT%3APSS+piezoresistive+wireless+biosensor%5BTitle%5D)+AND+%22Nano+Energy%22%5BJournal%5D)">CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor.</a> Nano Energy. 82, 105711(2021).</li>
<li>Zhang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+and+sensitive+detection+of+toxigenic+Fusarium+asiaticum+integrating+recombinase+polymerase+amplification%2C+CRISPR%2FCas12a%2C+and+lateral+flow+techniques%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Rapid and sensitive detection of toxigenic Fusarium asiaticum integrating recombinase polymerase amplification, CRISPR/Cas12a, and lateral flow techniques.</a> Int J Mol Sci. 24 (18), 14134(2023).</li>
<li>Manzo-Sánchez, G., Orozco-Santos, M., Martínez-Bolaños, L., Garrido-Ramírez, E., Canto-Canche, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Diseases+of+quarantine+and+economic+importance+in+banana+tree+%28Musa+sp.%29+in+M%C3%A9xico%5BTitle%5D)+AND+%22Rev+Mex+Fitopatol%22%5BJournal%5D)">Diseases of quarantine and economic importance in banana tree (Musa sp.) in México.</a> Rev Mex Fitopatol. 32 (2), 89-107 (2014).</li>
<li>Blomme, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Bacterial+diseases+of+bananas+and+enset%3A+Current+state+of+knowledge+and+integrated+approaches+toward+sustainable+management%5BTitle%5D)+AND+%22Front+Plant+Sci%22%5BJournal%5D)">Bacterial diseases of bananas and enset: Current state of knowledge and integrated approaches toward sustainable management.</a> Front Plant Sci. 8, 1290(2017).</li>
</ol>

<table><tbody><tr><td>EnGen lba Cas12a (Cfp1) 단백질</td><td>뉴잉글랜드 바이오랩스</td><td>M0653T</td><td></td></tr><tr><td>EnGen lba Cas12a 희석제</td><td>뉴잉글랜드 바이오랩스</td><td>B0653A</td><td></td></tr><tr><td>에펜도르프 PCR 튜브</td><td>머크</td><td>EP0030124332</td><td></td></tr><tr><td>에펜도르프 세이프락 마이크로 시험관</td><td>머크</td><td>EP0030121708</td><td></td></tr><tr><td>추출 백 유니버설</td><td>비오레바</td><td>430100</td><td></td></tr><tr><td>GeneRuler 100 bp DNA 사다리</td><td>써모 피셔 사이언티브</td><td>SM0241 / SM0242</td><td></td></tr><tr><td>지니 원심분리기 미니-6KO</td><td>옵티진</td><td>옵-퓨지</td><td></td></tr><tr><td>지니 III</td><td>옵티진</td><td>https://www.optigene.co.uk/instruments/genie-iii/&nbsp;</td><td></td></tr><tr><td>지니 스트립 홀더</td><td>옵티진</td><td>GBLOCK-01 / GBLOCK-02 / GBLOCK-03</td><td></td></tr><tr><td>지니 스트립</td><td>옵티진</td><td>OP-0008-50 / OP-0008-500</td><td></td></tr><tr><td>HiScribe T7 고수율 RNA 합성 키트</td><td>뉴잉글랜드 바이오랩스</td><td>E2040S</td><td></td></tr><tr><td>NanoDrop ND-1000 자외/비스 분광광도계</td><td>써모 피셔 사이언티브</td><td>https://caeonline.com/buy/spectrometers/nanodrop-nd-1000/293669253</td><td></td></tr><tr><td>NEBuffer 3</td><td>뉴잉글랜드 바이오랩스</td><td>B7003S</td><td></td></tr><tr><td>NEBuffer r2.1</td><td>뉴잉글랜드 바이오랩스</td><td>B6002S</td><td></td></tr><tr><td>폴리비닐피롤리돈(PVP) 분말</td><td>시그마-올드리치</td><td>9003-39-8</td><td></td></tr><tr><td>RNA 클린 및 농축기-5 키트</td><td>자이모 리서치</td><td>R1013</td><td></td></tr><tr><td>RNA 로딩 염료</td><td>써모 피셔 사이언티브</td><td>R0641</td><td></td></tr><tr><td>스마트블루 블루라이트 트랜스빌리미네이터</td><td>아큐리스 인스트루먼트</td><td>E4000-E</td><td></td></tr><tr><td>수산화나트륨(NaOH) 펠릿</td><td>머크, 엠파르타</td><td>K277</td><td></td></tr><tr><td>트리스(하이드록실메틸) 아미노메탄</td><td>머크</td><td>77-86-1</td><td></td></tr><tr><td>트위스탬프 리퀴드 기본 키트</td><td>트위스트 닥스</td><td>TALQBAS01</td><td></td></tr></tbody></table>

fusarium oxysporum f. sp. cubense tropical race 4를 검출하기 위한 복합 재조합효소 중합효소 증폭 crispr/cas12a 분석

Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4

Fusarium oxysporum f. sp. cubense Tropical Race 4를 검출하기 위한 복합 재조합효소 중합효소 증폭 CRISPR/Cas12a 분석

Regular and accurate surveillance stands central to the efficient management of plant diseases. It can indicate which course of action is most ...

combined-recombinase-polymerase-amplification-crisprcas12a-assay-for

Research

연구

JoVE Journal

Environment

환경

DNA 조립체를 사용하여, 여러 CRISPR 벡터가 CRISPR 다수의 구성을 하나 복제 반응에 병렬로 구성 될 수있는 간단한 작업 벡터. 토마토 털이 뿌리는 CRISPR 경로를 확인하고 돌연변이 물질을 생성 할 수있는 훌륭한 모델 시스템입니다.

토마토 털이 뿌리의 생성에 의한 높은 처리량 CRISPR 벡터 건설 및 DNA 수정의 특성

유전자 Ustilago이 maydis 검댕 곰팡이 조작에 우리는 강력한 유전자 교체 전략을 설명합니다. 이 프로토콜은 감염 표현형을 조사하기 위해 삭제 돌연변이를 생성하는 방법에 대해 설명합니다. 형광 표지 단백질을 코딩하는 서열을 추가하여, 예를 들어, 임의의 방법으로 유전자를 수정하도록 확장 될 수있다.

플랜트 병원체의 유전자 조작<em&gt; Ustilago maydis</em&gt; 곰팡이 생물과 식물 미생물 상호 작용을 연구하는

여기, 우리는 CRISPR-Cas9 ribonucleoproteins 및 homology 종속 복구 서식 파일을 사용 하 여 C. 선 충 의 게놈을 엔지니어링 하는 방법 제시.

빠른 고 손쉬운 파이프라인 C. 선 충 을 사용 하 여 CRISPR/Cas9 Ribonucleoproteins에서 게놈 포인트 돌연변이 생성에 대 한

이 프로토콜의 목표는 조립된 리보뉴클레오단백질 복합체와 스크리닝을 위한 dpy-10 공동 CRISPR 마커를 사용하여 C. elegans 게놈의 원활한 CRISPR/Cas9 편집을 가능하게 하는 것입니다. 이 프로토콜은 삽입, 삭제, 유전자 대체 및 코돈 대체를 포함하여 C. elegans에 있는 유전 수정의 각종을 만들기 위하여 이용될 수 있습니다.

CRISPR/Cas9 Dpy-10 공동 CRISPR 스크리닝 마커 및 조립된 리보뉴클레오단백질 복합체를 사용하여 C. elegans rbm-3.2 유전자의 편집.

이 연구에서는 CRISPR-Cas12a와 결합된 재조합 중합효소 증폭을 기반으로 하는 칸디다투스 리베리박터 아시아티쿠스에 대한 빠르고 민감하며 휴대 가능한 검출 방법이 개발되었습니다.

CRISPR-Cas12a와 결합된 재조합 중합효소 증폭을 사용한 현장 배치 가능 칸디다투스 리베리박터 아시아티쿠스 검출

CRISPR-Cas 시스템과 anti-CRISPR 단백질은 Saccharomyces cerevisiae의 부울 게이트 체계에 통합되었습니다. 새로운 소형 논리 회로는 우수한 성능을 보였으며 dCas9/dCas12a 기반 전사 인자와 항-CRISPR 단백질의 특성에 대한 이해를 심화시켰습니다.

CRISPR-Cas 시스템 및 Anti-CRISPR 단백질을 기반으로 하는 유전자 디지털 회로

DNA 바이러스의 미량 검출을 위한 CRISPR/Cas12a 시스템과 재조합 중합효소 증폭을 결합한 프로토콜을 제시하고, 현장 진단 DNA 바이러스 검출을 위한 인공 지능 지원 분류를 통해 휴대용 스마트폰 현미경을 구축합니다.

RPA-CRISPR/Cas12a-SPM 및 딥러닝 기반의 DNA 바이러스 검출 시스템

여기에서는 감염성 질환 병원체의 핵산 바이오마커 또는 기타 관심 유전자 마커의 검출에 사용할 수 있는 사전 혼합, 동결건조 재조합 효소 기반 등온 증폭 및 CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats) 기반 반응의 단계별 준비를 제시합니다.

Premixed and Freeze-dryed Reagents를 사용한 현장 진단 CRISPR 기반 진단

Acinetobacter baumannii의 빠르고 정확한 검출을 촉진하기 위해 A. baumannii 감염을 식별하기 위해 LbaCas12a 엔도뉴클레아제와 함께 Recombinase Polymerase Amplification(RPA)을 사용하는 프로토콜을 제시합니다.

Recombinase Polymerase Amplification/Cas12a 기반 시스템을 사용한 Acinetobacter Baumannii 감염의 빠르고 특이적 검출

여기에서는 증폭 준비 세포 용해, 재조합효소 중합효소 증폭 및 CRISPR/Cas12a 분석을 사용하여 Lotmaria passim 및 Nosema ceranae 와 같은 꿀벌 병원체에서 DNA를 검출하는 간단하고 빠른 절차를 설명합니다.

RPA/CRISPR/Cas12a 시스템과 결합된 알칼리 용해를 통한 트리파노소마티드 기생충 및 노세마 세라네 의 현장 DNA 검출

학습하세요 Fusarium oxysporum f. sp. cubense Tropical Race 4를 검출하는 CRISPR/Cas12a 분석법을 배워보세요. 재조합효소 중합효소 증폭과 결합하여 신속하고 경제적인 현장 진단이 가능합니다.

Fusarium oxysporum f. sp. cubense Tropical Race 4를 검출하기 위한 복합 재조합효소 중합효소 증폭 CRISPR/Cas12a 분석

더 많은 동영상 탐색