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Methods Collections

State-of-the-art methods to study adipocytes and adipose tissue

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Methods Collections
State-of-the-art methods to study adipocytes and adipose tissue

Guest Editors
Jerome Gilleron

Université Côte d'Azur

Dr. Jerome Gilleron earned his Ph.D. on “cell signaling, endocrinology, and reproduction” from the University …

Jennifer Jager

Université Côte d'Azur

Dr. Jennifer Jager received her Ph.D. in Biology from the University of Nice-Sophia Antipolis (France) in 2009, where she …

Collection Overview

Obesity has become a major worldwide public health issue, dramatically increasing the risk of developing cardiovascular disease, type 2 diabetes, and liver diseases. Obesity is characterized by an increase in adipose tissue (AT) mass, as well as a tremendous remodeling of AT immune cells from anti-inflammatory toward pro-inflammatory status. It is now clear that the maximal capacity of AT to expand during obesity is a key factor in the development of obesity-associated pathologies: AT expansion is an important homeostatic mechanism to cope with the excess of energy and to avoid deleterious lipid spill over in other metabolic organs such as muscles and the liver. Understanding (during obesity) (1) how AT expand; (2) which are the structural and cellular remodeling that leads to the failure in AT expansion; and (3) how these defects can be corrected are fundamental questions that must be resolved in order to pave the way for therapeutic approaches. Recently, several elegant methods were developed both in vitro and in vivo to determine the molecular mechanisms that tune adipogenesis, adipocyte growth and function, AT development and AT cellular remodeling. This methods collection aims to coalesce these various innovative techniques and tools in order to explore AT in physiological and pathological conditions, and ultimately bring our understanding of this tissue and its important pathological complications to a clinical level.

 

Articles

Abstracts

ADIPOCYTE 3D CULTURE AS A MODEL TO STUDY CACHEXIA-INDUCDE BEIGE REMODELING OF WHITE ADIPOSE TISSUE

Miguel Batista Junior*1, Stephen Farmer2
1Universidade de Mogi das Cruzes, 2Boston University School of Medicine

Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

Jonquil Poret, MS*1, Patricia Molina, MD, PhD1, Liz Simon, MVSc, PhD*1
1Louisiana State University Health Sciences Center - New Orleans

Human and murin cell culture models to manipulate proteins and non-coding RNAs expression and study its impact on adipocyte functions.

Jennifer Jager*1
1Université Côte d'Azur, Institut National de la Santé et de la Recherche Médicale (INSERM) U1065, Centre Méditerranéen de Médecine Moléculaire (C3M), France

The Isolation and Characterisation of Primary Human Adipocyte Derived Extracellular Vesicle

Naveed Akbar*1
1University of Oxford

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