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TOPICAL COLLECTIONS

Environmental Molecular Biology Tools for Monitoring and Advancing Bioremediation
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Guest Editor

Kate Kucharzyk

Kate Kucharzyk

Battelle Memorial Institute

<p>Dr. Kate Kucharzyk is a research leader at Battelle’s National Security CBRNE business line, where she has applied over a decade of expertise in biochemistry, environmental microbiology, and omics. Her work integrates chemistry, biology, and materials science to develop microbially based solutions for national security and environmental challenges. Since joining Battelle in 2014, she has led numerous multidisciplinary programs for agencies, including the DOD, DOE, DARPA, and EPA. In 2018, she helped establish Bioenabled Solutions (BIOS), a cross-disciplinary research capability supporting synthetic biology, and now serves as its technical leader, guiding strategy across multiple focus areas.&nbsp;</p><p><br></p><p>Dr. Kucharzyk holds an M.S. in Biochemistry from Maria Curie University, a PhD in Environmental Microbiology and Biochemistry from the University of Idaho, and completed postdoctoral training in geomicrobiology at Duke University. She has authored over 30 papers, holds four patents, and is an active member of ACS, ASM, ITRC, and the Battelle Technical Council.</p>

Collection Overview

Environmental molecular biology tools (EMBT) are essential for evaluating the performance and functionality of biotechnology-based solutions. Whether applied in the field or at the microcosm scale, EMBTs provide direct evidence of ongoing biological processes that are often overlooked by practitioners but can significantly aid in achieving program goals.


Some good examples of EMBTs include:

1. Discovery and Targeted Proteomics: These techniques resolve protein-level information in a sample, allowing us to confirm whether biocatalysis is occurring.

2. Sequencing: Both whole genome and 16S sequencing can identify genes and microbial species present in a sample.

3. Metabolomics: This method analyzes small molecules, helping to attribute their presence to specific biological processes.


This JoVE topical collection focuses on EMBT methodologies, particularly those that can be readily applied to evaluate bioremediation activities.

Articles

Microbial DNA Analysis in the Field Using a Biological Extraction Field Kit and a Field qPCR Unit
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Microbial DNA Analysis in the Field Using a Biological Extraction Field Kit and a Field qPCR Unit

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Abstracts

Application of carbon-14 assays to measure rate constants for degradation of environmental pollutants

David Freedman*1

1Clemson University

Construction and Validation of an E. coli Whole-Cell Biosensor Based on CadC and GFP for Heavy Metal Detection

Dilani Dissanayake*1

1Department of Chemistry, Faculty of Science, University of Colombo, Colombo 00300, Sri Lanka.