
Environmental molecular biology tools (EMBT) are essential for evaluating the performance and functionality of biotechnology-based solutions. Whether applied in the field or at the microcosm scale, EMBTs provide direct evidence of ongoing biological processes that are often overlooked by practitioners but can significantly aid in achieving program goals.
Some good examples of EMBTs include:
1. Discovery and Targeted Proteomics: These techniques resolve protein-level information in a sample, allowing us to confirm whether biocatalysis is occurring.
2. Sequencing: Both whole genome and 16S sequencing can identify genes and microbial species present in a sample.
3. Metabolomics: This method analyzes small molecules, helping to attribute their presence to specific biological processes.
This JoVE topical collection focuses on EMBT methodologies, particularly those that can be readily applied to evaluate bioremediation activities.
Application of carbon-14 assays to measure rate constants for degradation of environmental pollutants
David Freedman*1
1Clemson University
Construction and Validation of an E. coli Whole-Cell Biosensor Based on CadC and GFP for Heavy Metal Detection
Dilani Dissanayake*1
1Department of Chemistry, Faculty of Science, University of Colombo, Colombo 00300, Sri Lanka.