Methods Collections

Protein-Protein Interaction Methods

Guest Editors: Alastair Barr, Michael Overduin
The study of protein-protein interactions is key to understanding the network of molecular interactions underlying biological effects, and defining how individual proteins perform their functional role within a cell.  Detailed understanding of t Show More

Analysis of Plant Acyl Lipids

Guest Editors: Jenny Mortimer, Toshiki Ishikawa
Plant acyl lipids have many different functions which include compartmentalization, energy storage, signaling, drought tolerance and pathogen resistance. Classes of acyl lipids include the sphingolipids, phospholipids, and glycerolipids, and a high d Show More

Methods for Studying Neuroinflammation in Rodent and Model Systems

Guest Editors: Tammy Butterick, Joshua Nixon, Cayla Duffy
Chronic neuroinflammation is known to be heightened in obesity and neurodegenerative diseases such as Alzheimer’s disease (AD). Microglia, the resident immune cells of the brain, are highly dynamic cells that mediate brain immune responses, mai Show More

Mechanical Stimulation Techniques

Guest Editor: Pushkar Lele
A significant body of research suggests that extracellular mechanical cues influence important biological processes, including intracellular signaling, genetic regulation, division and motility. Such mechanical effects are prominent in eukaryotes as Show More

Quantitative and Live Imaging Techniques for Studying Developmental Biology

Guest Editor: Laina Freyer
The ability to visualize a process and capture it in real-time can sometimes be the distinguishing factor in understanding mechanisms of cell behavior and tissue morphogenesis. Time-lapse imaging of organisms, tissues, and cells allows us to directly Show More

Functional Analysis of Human p53 Mutations and Interactions in Yeast Cells

Guest Editors: Daria Hekmat-Scafe, Tim Stearns
The tumor suppressor, p53, plays a central role in cancer and presents an attractive target for drug development. Mutations in the TP53 gene have been observed in ~50% of human tumors, and perturbation of components of the p53 network have been impli Show More

Methods and Techniques for Micro and Electro-Propulsion for Cubesats and Smart Satellites 

Guest Editors: Igor Levchenko, Angelo Cervone, St├ęphane Mazouffre, Mitchel Walker, Shuyan Xu
Rapid development of information technologies and material science has formed a unique base for the space exploration renaissance. Miniaturization of electronic and energy generating devices, along with novel materials and innovative paradigms in roc Show More

Nanochemistry for Photonics Applications

Guest Editor: Roshini Ramachandran
The field of nanotechnology is rapidly advancing to encompass a wide variety of uses in photonics. The structures of nanomaterials are carefully synthetically tailored to subsequently tune their properties for specialized applications. Owing to the e Show More

Functional genomic methods to interrogate global mechanisms of gene regulation

Guest Editor: Bryan Venters
The mechanisms by which the transcription machinery assembles and operates in the context of chromatin have been the focus of intense study for the last several decades. It is becoming increasingly clear that integrative analyses of high-quality Show More

Chloroplast research methods: probing the localization, topology, targeting and interactions of chloroplast proteins

Guest Editor: Paul Jarvis
Chloroplasts are the organelles that define plants. Along with many other metabolic, developmental and signalling functions, chloroplasts are responsible for photosynthesis – the process whereby sunlight energy is harnessed to power the cellula Show More

Methods and Techniques for the Development of Drug Delivery Systems

Guest Editor: Wing-Fu Lai
As far as pharmaceutical interventions are concerned, one of the important factors to be considered is drug bioavailability, which can be easily jeopardized by various factors (particularly the physiological barriers and blood clearance) after drug a Show More