Methods Collections

Protein-Protein Interaction Methods

Guest Editors: Alastair Barr, Michael Overduin
The study of protein-protein interactions is key to understanding the network of molecular interactions underlying biological effects, and defining how individual proteins perform their functional role within a cell.  Detailed understanding of t Show More

Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control

Guest Editor: Charles E. Rupprecht, VMD MS PhD
Rabies is an acute progressive encephalitis caused by a lyssavirus. The case fatality of this zoonosis is the highest for any infectious disease. Although laboratory techniques on rabies have progressed greatly over the last century, typically such i Show More

Comprehensive Guide to Inflammation Detection Methodologies

Guest Editor: Payel Sil
Inflammation is the natural response of the body to harmful stimuli and inflammatory symptoms are routinely used to diagnose ailments. However, acute and chronic inflammation display differing characteristics, which requires deeper exploration for ac Show More

Mass Spectrometry Imaging, From Tissues to Single Cells

Guest Editors: Matthew O'Rourke, Matthew Padula, Greg Sutherland
This collection is focused on the techniques used for preparing samples for the different forms of mass spectrometry imaging (MSI) as well as any novel data processing pipelines. The aim is to demonstrate workflows that are not well known and that ar Show More

Current methods in Astyanax mexicanus research

Guest Editors: Misty Rose Riddle, Clifford J. Tabin
The Mexican Tetra, Astyanax mexicanus is an excellent model organism to study the developmental and genetic basis of evolution. This species of fish consists of a river-dwelling (surface) population and a number of cave-dwelling po Show More

Studying lipid-protein interactions

Guest Editor: Balaji Olety
Peripheral and integral membrane proteins that interact with lipids make up nearly one third of the total proteome. Despite its abundance, the mechanisms of their interactions with lipid moieties are well understood only for a fraction of them. This Show More

Methods and Techniques for the Development of Drug Delivery Systems

Guest Editor: Wing-Fu Lai
As far as pharmaceutical interventions are concerned, one of the important factors to be considered is drug bioavailability, which can be easily jeopardized by various factors (particularly the physiological barriers and blood clearance) after drug a Show More

Analysis of Plant Acyl Lipids

Guest Editors: Jenny Mortimer, Toshiki Ishikawa
Plant acyl lipids have many different functions which include compartmentalization, energy storage, signaling, drought tolerance and pathogen resistance. Classes of acyl lipids include the sphingolipids, phospholipids, and glycerolipids, and a high d Show More

Mechanical Stimulation Techniques

Guest Editor: Pushkar Lele
A significant body of research suggests that extracellular mechanical cues influence important biological processes, including intracellular signaling, genetic regulation, division and motility. Such mechanical effects are prominent in eukaryotes as Show More

Methods and Techniques for Micro and Electro-Propulsion for Cubesats and Smart Satellites 

Guest Editors: Igor Levchenko, Angelo Cervone, St├ęphane Mazouffre, Mitchel Walker, Shuyan Xu
Rapid development of information technologies and material science has formed a unique base for the space exploration renaissance. Miniaturization of electronic and energy generating devices, along with novel materials and innovative paradigms in roc Show More

Chloroplast research methods: probing the localization, topology, targeting and interactions of chloroplast proteins

Guest Editor: Paul Jarvis
Chloroplasts are the organelles that define plants. Along with many other metabolic, developmental and signalling functions, chloroplasts are responsible for photosynthesis – the process whereby sunlight energy is harnessed to power the cellula Show More

Methods for the Assessment of Mitochondrial Bioenergetics and Functions.

Guest Editor: Ryan J. Mailloux, PhD
Mitochondria are the main source of ATP in most, if not all, mammalian cells. However, these dynamic organelles fulfill a myriad of other cell functions including providing antioxidant support and reactive oxygen species to the cell, serving as an im Show More