Methods Collections

Protein-Protein Interaction Methods

Guest Editors: Alastair Barr, Michael Overduin
The study of protein-protein interactions is key to understanding the network of molecular interactions underlying biological effects, and defining how individual proteins perform their functional role within a cell.  Detailed understanding of t Show More

Accelerating the Development of Extracellular Vesicles for Therapeutic Use

Guest Editors: W. Keith Jones, Simon Kaja
Historically, extracellular vesicles (EVs) were considered merely a cellular system to recycle or eliminate intracellular components. It is now recognized that EVs are critical for intercellular communication at an organismal level. EVs are a heterog Show More

Responses of Livestock and Wildlife Species to Zoonotic Bacteria

Guest Editor: Catherine Vrentas
Due in large part to the immense potential of genetic manipulation in rodent systems, immunological investigations in mouse models have been used to reveal the intricacies of innate and acquired mammalian immune systems.  Similarly, mice and rat Show More

Methods to Measure Functional Beta Cell Mass

Guest Editors: Jeffrey Tessem, Patrick Fueger
The beta cell plays an essential role in physiology. These cells, found in the pancreatic Islets of Langerhans, must sense elevated circulating blood glucose levels and secrete the hormone insulin to cause the organism to clear elevated glucose Show More

Materials and Methods for the Development of Optical and Ultrasound Phantoms 

Guest Editor: Wenfeng Xia
Phantoms are widely used for performance evaluation of biomedical imaging systems, for training clinical practitioners, and for surgical planning. Materials that are suitable for phantom development are required to simulate specific physical properti Show More

Techniques for the Detection of Early Stage Prostate Cancer

Guest Editors: Fubo Wang, Huan Xu
This Methods Collection focuses on the early detection and diagnosis of prostate cancer (PCa). PCa is the second most frequently diagnosed cancer overall and is the fifth leading cause of cancer mortality in men. Prognosis is relatively positive if t Show More

Viral and Non-Viral Delivery Methods of CRISPR/Cas9 Toolbox

Guest Editors: Alessandra Recchia, Daniela Benati
CRISPR/Cas genome editing systems represent one of the most robust platforms in basic biomedical research and therapeutic applications. To date, the strategies of CRISPR/Cas9 delivery are primarily physical/chemical approaches such as transfecti Show More

Methods for the Assessment of Mitochondrial Bioenergetics and Functions

Guest Editor: Ryan J. Mailloux
Mitochondria are the main source of ATP in most, if not all, mammalian cells. However, these dynamic organelles fulfill a myriad of other cell functions including providing antioxidant support and reactive oxygen species to the cell, serving as an im Show More

Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control

Guest Editor: Charles E. Rupprecht
Rabies is an acute progressive encephalitis caused by a lyssavirus. The case fatality of this zoonosis is the highest for any infectious disease. Although laboratory techniques on rabies have progressed greatly over the last century, typically such i Show More

Comprehensive Guide to Inflammation Detection Methodologies

Guest Editor: Payel Sil
Inflammation is the natural response of the body to harmful stimuli and inflammatory symptoms are routinely used to diagnose ailments. However, acute and chronic inflammation display differing characteristics, which requires deeper exploration for ac Show More

Current methods in Astyanax mexicanus research

Guest Editors: Misty Rose Riddle, Clifford J. Tabin
The Mexican Tetra, Astyanax mexicanus is an excellent model organism to study the developmental and genetic basis of evolution. This species of fish consists of a river-dwelling (surface) population and a number of cave-dwelling po Show More

Methods and Techniques for the Development of Drug Delivery Systems

Guest Editor: Wing-Fu Lai
As far as pharmaceutical interventions are concerned, one of the important factors to be considered is drug bioavailability, which can be easily jeopardized by various factors (particularly the physiological barriers and blood clearance) after drug a Show More

Methods and Techniques for Micro and Electro-Propulsion for Cubesats and Smart Satellites 

Guest Editors: Igor Levchenko, Angelo Cervone, Stéphane Mazouffre, Mitchel Walker, Shuyan Xu
Rapid development of information technologies and material science has formed a unique base for the space exploration renaissance. Miniaturization of electronic and energy generating devices, along with novel materials and innovative paradigms in roc Show More

Chloroplast research methods: probing the localization, topology, targeting and interactions of chloroplast proteins

Guest Editor: Paul Jarvis
Chloroplasts are the organelles that define plants. Along with many other metabolic, developmental and signalling functions, chloroplasts are responsible for photosynthesis – the process whereby sunlight energy is harnessed to power the cellula Show More

Current Methods in Surgical Treatment for Primary Colorectal Cancer

Guest Editors: Al-guo Lu, Wolfgang Erwin Thasler , Feng Hao
Surgery is currently the most appropriate choice of curative treatment for primary colorectal cancer.  The current management is a complete mesocolic excision (CME) for colonic cancer or a total mesorectal excision (TME) of the rectum to remove Show More