Methods Collections

Protein-Protein Interaction Methods

Guest Editors: Alastair Barr, Michael Overduin
The study of protein-protein interactions is key to understanding the network of molecular interactions underlying biological effects, and defining how individual proteins perform their functional role within a cell.  Detailed understanding of t Show More

RNA/DNA In Situ Hybridization techniques

Guest Editor: Irinna Papangeli
In Situ Hybridization (ISH) provides a powerful tool for localized detection of RNA/DNA sequences, widely applied in both research and clinical contexts. This technique takes advantage of nucleic acid probes that are complementary to target RNA or DN Show More

Minimally Invasive Pancreatic Surgery 

Guest Editors: Marc Besselink, Mohammed Abu Hilal, Ugo Boggi, Safi Dokmak, Amer Zureikat, Maarten Korrel, Frederique Vissers, Sanne Lof
Minimally invasive pancreatic surgery is on the rise worldwide. Minimally invasive distal pancreatectomy (MIDP) has been reported to be superior to open distal pancreatectomy (ODP) in terms of blood loss and hospital stay. The oncological safety of M Show More

Techniques for high throughput lifespan measurement in invertebrate model systems

Guest Editor: Jonathan Millen
Recently, a great deal of effort has been focused on longevity and aging. In particular, new treatments for age-related diseases and the extension of the human healthspan are the subject of much this attention. A major inhibitor of progress towards b Show More

Prostate Organoid Research Techniques and Methods: Three-Dimensional (3D) Culture, Spin-down, Dimpled Parafilm and Fusion Method

Guest Editors: Christina Jamieson, Sanghee Lee
Organoids are three-dimensional (3D) ex vivo cellular structures that are believed to recapitulate and maintain the in vivo conditions of cellular viability and responsiveness to therapeutic drugs. Matrigel or collagen have been utilized as an extrac Show More

New Techniques and Approaches for the Assessment or Intervention in Learning Disabilities and ADHD

Guest Editors: Celestino Rodríguez, Débora Areces, Marisol Cueli, Trinidad García
International classifications of mental disorders for the diagnosis of Learning Disabilities (LD) and Attention Deficit/Hyperactivity Disorders (ADHD) establish that they are not the result of other disabling conditions, although they might occur con Show More

Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control

Guest Editor: Charles E. Rupprecht
Rabies is an acute progressive encephalitis caused by a lyssavirus. The case fatality of this zoonosis is the highest for any infectious disease. Although laboratory techniques on rabies have progressed greatly over the last century, typically such i Show More

Comprehensive Guide to Inflammation Detection Methodologies

Guest Editor: Payel Sil
Inflammation is the natural response of the body to harmful stimuli and inflammatory symptoms are routinely used to diagnose ailments. However, acute and chronic inflammation display differing characteristics, which requires deeper exploration for ac Show More

Current methods in Astyanax mexicanus research

Guest Editors: Misty Rose Riddle, Clifford J. Tabin
The Mexican Tetra, Astyanax mexicanus is an excellent model organism to study the developmental and genetic basis of evolution. This species of fish consists of a river-dwelling (surface) population and a number of cave-dwelling po Show More

Methods and Techniques for the Development of Drug Delivery Systems

Guest Editor: Wing-Fu Lai
As far as pharmaceutical interventions are concerned, one of the important factors to be considered is drug bioavailability, which can be easily jeopardized by various factors (particularly the physiological barriers and blood clearance) after drug a Show More

Chloroplast research methods: probing the localization, topology, targeting and interactions of chloroplast proteins

Guest Editor: Paul Jarvis
Chloroplasts are the organelles that define plants. Along with many other metabolic, developmental and signalling functions, chloroplasts are responsible for photosynthesis – the process whereby sunlight energy is harnessed to power the cellula Show More

Multi-modal Analytics in Energy Storage

Guest Editors: Partha P. Mukherjee, Leela M. R. Arava, George J. Nelson
Energy storage is a critical enabler for vehicle electrification and renewable energy integration into the electric grid. Primary challenges in lithium based intercalation (Li-ion) and conversion (e.g., Li-S) chemistries continue to remain in en Show More