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TOPICAL COLLECTIONS

Approaches for Site-specific Protein Modification

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Guest Editors

Louis Luk

Louis Luk

Cardiff University School of Chemistry

<p>Louis YP&nbsp;Luk&nbsp;is a Lecturer at the School of Chemistry of Cardiff University. He obtained his BSc in Chemistry and Microbiology &amp; Immunology at the University of British Columbia. He obtained his PhD in the same university under the supervisor of Martin E. Tanner, followed by post-doctoral studies in the laboratories of Stephen BH Kent at the University of Chicago and Rudolf K. Allemann at Cardiff University. Louis became a University Research Fellow in 2015 and was recently tenured at Cardiff University. Combining his training in enzymology and peptide chemistry, Louis&#39; current research focuses on artificial enzyme design and protein bioconjugation chemistry.&nbsp;&nbsp;</p>

Yu-Hsuan Tsai

Yu-Hsuan Tsai

Cardiff University

<p>Dr. Tsai is a lecturer in the Cardiff School of Chemistry. He obtained his BSc at the National Taiwan University (2006), MSc at the ETH Zurich (2008), and Dr rer. nat. at the Freie Universit&auml;t Berlin (2012). He was a graduate student under the guidance of Peter H. Seeberger at the ETH Zurich and Max Planck Institute of Colloids and Interfaces, and a postdoctoral researcher in the Jason W. Chin group at the MRC Laboratory of Molecular Biology. As an independent researcher in Cardiff, his research focuses on developing tools to study and control protein functions.</p>

Collection Overview

Proteins are important biomolecules to sustain life-they perform a wide array of critical roles in natural organisms and biological systems. Over the previous decades, advances in technology have allowed us to modify proteins in very targeted ways, which opens up an even larger range of tailored functions for specific desired applications. Therefore, approaches for the selective modification of proteins are indispensable in life science and biomedical research. This Methods Collection will highlight video articles documenting innovative and gold-standard methods, and will cover topics in: enzymatic approaches for protein modifications, chemical approaches (including bioorthogonal reactions) for protein modifications, chemical genetic approaches for protein modulation, and site-specific protein modification for biological applications.

Articles

Site-Specific Lysine Lactylation via Genetic Code Expansion in <em>E. coli </em>and Mammalian Cells
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Site-Specific Lysine Lactylation via Genetic Code Expansion in E. coli and Mammalian Cells

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2026

Abstracts

<p>Organocatalyst-mediated protein aldol ligations (OPAL) of proteins</p>

Martin Fascione*1

1University of York

<p>Preparation of site-specific cytotoxic protein conjugates via maleimide-thiol chemistry and sortase A-mediated ligation.</p>

Lukasz Opalinski*1

1Departmentof Protein Engineering, Faculty of Biotechnology, University of Wroclaw