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Methods Collections

Current methods in chick embryology

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Methods Collections
Current methods in chick embryology

Guest Editors
Masahito Yamagata

Department of Molecular and Cellular Biology, Center for Brain Science, Harvard University

Dr. Masahito  is an associate at the Department of Molecular and Cellular Biology, Center for Brain Science…

Collection Overview

In recent decades, the application of new molecular and cellular techniques in avian embryos has opened up new avenues to understanding various biological questions in birds, including chickens (Gallus gallus). Questions include, how cells grow, migrate, differentiate, and function, and how birds behave. These techniques may also be beneficial to existing industries, such as poultry and medicine. For example, emerging single-cell RNA sequencing and related multimodal molecular approaches have led to the description of new cell types and their states. Electroporation, viral vectors, and ever-improving genome editing methods using CRISPR, have allowed us to inquire into the functions of particular genes and visualize specific cell populations in tissues and animals. Chicken eggs are also essential resources for the production of IgY antibodies and vaccines for infectious diseases. This Methods Collection will focus on new techniques with which to study chicken or other avian embryos.

Articles

Abstracts

A simplified method for isolation and differentiation of preadipocytes from broiler chick embryos

Minjeong Kim1
1University of Tennessee

En-face culture of the avian embryonic neuroepithelium for live imaging of fluorescent reporters

Xavier Morin*1, Rosette Goïame1, Evelyne Fischer1, Samuel Tozer1
1Institut de Biologie de l'ENS (IBENS), Ecole Normale Supérieure, CNRS, INSERM, Université PSL, 75005 Paris, France.

Tonotopic Gradient and Microcircuits in Chicken Embryo Auditory Brainstem Slices

Sandesh Mohan*1, Abhijit Roy2, Jason Tait Sanchez2
1Norhwestern University, 2Northwestern University

Important Factors Influencing the Recording of Chicken Auditory Brainstem Responses

George Ordiway1, Miranda McDonnell1, Jason Tait Sanchez*1
1Northwestern University

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