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Chapters
- 00:05Introduction
- 01:03Adipose Tissue Collection and Digestion
- 02:21Digestion of Adipose Tissue
- 04:48Results: Comparison of Cell Morphology and Lipid Accumulation
- 05:47Conclusion
The present protocol describes a simple method for isolating preadipocytes from adipose tissue in broiler embryos. This method enables isolation with high yield, primary culture, and adipogenic differentiation of preadipocytes. Oil Red O staining and lipid/DNA stain measured the adipogenic ability of isolated cells induced with differentiation media.
