Inducing Traumatic Injury in a Brain Slice Using a Shock Tube Device

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Samenvatting

Source: Campos-Pires, R., et al. A novel in vitro model of blast traumatic brain injury. J. Vis. Exp. (2018)

In this video, a shock tube device is used to generate a shockwave by rupturing a polymer diaphragm through controlled pressurization. The shockwave impacts brain slices contained in a secured bag, inducing traumatic injury to the brain slice.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Hippocampal Organotypic Slice Preparation and Culture

NOTE: This protocol allows the production of organotypic hippocampal slices. Ideally, no more than three animals should be euthanized and dissected in one session to ensure each step is done swiftly and to avoid compromising the quality of the slices. Use an aseptic technique throughout.

Ensure the following steps are taken before starting the dissection pro....

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Resultaten

Pneumatic launcher diagram; control panel, sensors, oscilloscope for dynamic pressure study.
Figure 1: Schematic of the shock tube device with the sterile bag holder frame. (A). The shock tube is a 3.8 m long stainless-steel tube made of three 1.22 m long sections, connected by gaskets and flanges, with an internal diameter of 59 mm. (B) Inset shows th.......