JoVE Encyclopedia of Experiments
Neuroscience
0 views • 3:56 min • July 8th, 2025
Begin with a shock tube setup comprising a proximal driver section, a diaphragm breech assembly, and a distal driven section with pressure sensors and a sample holder.
The device is connected to a pressurized gas cylinder via the control unit.
Insert a blanking rod through the sample holder to align it with the shock tube outlet.
Attach the pressure sensors to a recording device.
Keep the shock tube control closed, and open the gas cylinder outlet to build pressure in the control unit.
Secure a polyester sheet diaphragm in the breech assembly.
Place a bag containing a membrane with brain slices in an experimental medium into the sample holder.
Open the gas control knob of the shock tube, allowing gas to enter the driver section and increase air pressure.
This ruptures the diaphragm, releasing high pressure that generates a shockwave.
This shockwave travels and impacts the brain slices, inducing traumatic tissue injury.
Use a blanking rod to bolt the sterile bag holder frame to the shock tube distal flange, ensuring that the central hole is aligned with the shock tube outlet. Sensor 1, a pressure transducer is located in the middle part of the driven section, and sensor 2 is in the
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