Methodenartikel

Intranasal Mesenchymal Stem Cell Delivery in a Mouse Model of Traumatic Brain Injury

17 juni 2025

In dit artikel

Samenvatting

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

Source: Shahror, R. A. et. al., Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model. J. Vis. Exp. (2019)

This video demonstrates intranasal mesenchymal stem cell (MSC) delivery in a mouse model. MSCs absorb into nasal tissue, migrate to the brain via cytokine signals, and secrete neurotrophic factors at the injury site, offering neurotherapeutic potential for neuronal repair.

Protocol

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Labeling of mesenchymal stem cells (MSCs) with superparamagnetic iron oxide (SPIO) Nanoparticles

  1. To label MSCs with SPIO, add 6 mL of labeling media (25 µg/mL of SPIO in Dulbecco's modified Eagle medium (DMEM) with no fetal bovine serum (FBS)) to a T75 flask containing MSCs (80% confluency).
  2. Incubate the cells with the labeling media in a CO2 incubator (37 °C, 5% CO2 ) without shaking. After 24 h, gently remove the labeling media using a sterile Pasteur pipette with a pl....

Toegang beperkt. Log in of start een proefperiode om deze inhoud te bekijken.

Resultaten

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,
MSC labeling and delivery process diagram with SPIO, MRI imaging, fluorescent microscopy results.

Figure 1: Schematic flowchart of the protocol and in vitro confirmation of SPIO uptake by MSCs. (A) MSCs were .......

Toegang beperkt. Log in of start een proefperiode om deze inhoud te bekijken.

Materialen

Lijst van materialen gebruikt in dit artikel
NaamBedrijfCatalogusnummerOpmerkingen
Cell culture supplies (Plastics)ThermoFisher ScientificVariesReplaceable with any source
Disposable microtome bladeVWR95057-832
D-MEM/F-12 (1X) with GlutaMAXGIBCO10565-018
Embedding medium for frozen tissue specimens (O. C. T.)Sakura Finetek4583
Fetal bovine serum (FBS)GIBCO12662-029
Fluorescence wild field microscopeOlympusOlympus BX43
ForcepFine Science Tools11293-00Surgery
Gentamicin (10 mg/mL)GIBCO15710-064
Hair clipperPet ClubPC-400
Head trauma contusion devicePrecision Systems and InstrumentationModel TBI-0310
Hyaluronidase from bovine testesMilliporeSigmaH3506
Ketamine (Ketavet)Pfizer778-551
MiceNational Laboratory Animal Center, TaiwanC57BL6Wild type mice strain used in the study
MicrodrillNakanishiNE50Combine with Burrs for generating the bone window
MicrotomeLeicaRM2265
Mouse (C57BL/6) mesenchymal stem cellsGIBCOS1502-100
MRI scannerBruker Biospec
Phosphate buffer saline (PBS)Corning Cellgro/ThermoFisher21-031-CV
Povidone-iodine 7.5%Purdue product L.P. Surgical scrub
ScissorFine Science Tools14084-08Surgery
Stereotaxic frameKopf InstrumentsModel 900
Superparamagnetic iron oxide (SPIO) nanoparticlesBioPALMolday ION EverGreen, CL-50Q02-6A-51Stem cells labeling for in vivo tracking using MRI
Suture monofilamentEthiconG697Suture
TimerWisewind Replaceable with any source
TrypLEGIBCO12604-013
Xylazine (Rompun)BayerQN05 cm92

Herprints en machtigingen

Toestemming aanvragen om de tekst of afbeeldingen van dit JoVE-artikel te hergebruiken

Toestemming aanvragen

Trefwoorden

Intranasale toedieningmesenchymale stamcellenhyaluronidasebehandelingMSC migratieneurotrofe factorencytokinesignalennasale absorptielocatie van hersenletsel

Gerelateerde artikelen