JoVE Encyclopedia of Experiments
Neuroscience
0 views • 2:39 min • June 17th, 2025
Begin with an anesthetized mouse with a traumatic brain injury.
At the injury site, axonal demyelination and neuronal damage trigger the release of pro-inflammatory cytokines.
Secure the mouse and intranasally deliver a proteolytic enzyme.
Next, position the mouse face up for a short duration and repeat the enzyme delivery.
The proteolytic enzymes degrade the extracellular matrix in the nasal epithelia and enhance the cell permeability.
Then, intranasally administer the labeled mesenchymal stem cell, or MSC solution.
Hold the mouse’s head upright for a while to prevent premature drainage and allow MSC absorption into the nasal tissue.
Repeat the MSC delivery process.
Allow the mouse to recover.
The chemotactic signals of pro-inflammatory cytokines direct MSCs to migrate from the nasal cavity to the brain injury site.
At the injury site, MSCs secrete neurotrophic factors that promote remyelination. This highlights MSCs’ neurotherapeutic potential for neuronal repair.
For cell delivery, after confirming a lack of response to toe pinch, scruff the mouse while immobilizing the skull. Place the tip of a pipette containing 4 units of hyaluronidase per microliter of PBS near the nare of the mouse a
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