Axonal Adhesion Assessment
Axonal Adhesion Assessment - Related Videos
09:31
Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
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Cited by 1 •
2013
We measured the tension release in an axon that was partially lesioned with a laser dissector by simultaneous force spectroscopy measurement performed on an optically-trapped probe adhered to the membrane of the axon. The developed experimental protocol evaluates the axon adhesion to the culture substrate.
02:44
In Vitro Gut Model to Assess Bacterial Adhesion and Internalization
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2026
Source: Calatayud Arroyo, M., et al. Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface. J. Vis. Exp. (2018).This video demonstrates an in vitro gut host–microbe model to study bacterial viability. A co-culture of Caco-2 and HT29-MTX cells is incubated with bacteria pre-treated under simulated digestive conditions. Epithelial barrier integrity is assessed using transepithelial electrical resistance, and fractions from the medium, solubilized...
01:14
Adhesion
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2019
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules. Capillary action is a result of water’s adhesive tendencies. When a narrow glass...
10:30
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
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Cited by 10 •
2014
We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.
03:24
Two-Photon Laser Axotomy: A Method to Injure Axons in Zebrafish Embryos and Observe Axonal Recovery
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2023
This video describes the method to injure axons in zebrafish embryos using two photon laser axotomy and observing axonal recovery from injury.
FAQs
Axonal adhesion reflects more than initial contact: attachment, spreading, and continued retention describe different outcomes of association with a surface. Adhesive cues within a biological or engineered substrate can influence whether axons remain associated over time. Separating these outcomes helps bioengineers determine whether a material supports only early association or also longer-term structural stability.
The measurement set should treat attachment, spreading, retention, and detachment as related but nonidentical outcomes. Strong initial attachment does not by itself show that axons remain associated with a candidate surface, while detachment indicates loss of that association under the tested conditions. Comparing these measures gives a more complete picture of substrate performance for neural tissue formation and repair.
Results depend on interactions between neuronal axons and the selected surface, including its adhesive cues and engineered design. Comparisons should hold testing conditions consistent while varying the biological substrate, biomaterial, coating, or scaffold feature of interest. This approach makes differences in measured attachment or retention easier to attribute to the candidate surface rather than to unrelated experimental changes.
An assessment generally begins by culturing neurons on one or more candidate substrates under controlled conditions. Researchers then quantify outcomes such as axon attachment, spreading, retention, or detachment and compare the results across the tested surfaces. Organizing the workflow around separate measurements connects the culture experiment to a specific question about material or scaffold performance.
Bioengineers can use Axonal Adhesion Assessment to compare biomaterials, surface coatings, and scaffold designs intended for nerve guidance, neural interfaces, or regenerative strategies. The most useful comparison depends on the outcome being prioritized: early attachment, axon spreading, continued retention, or resistance to detachment. This makes adhesion data relevant to both material selection and design refinement.
Adhesion measurements connect cell-material interactions with larger engineering goals. If a tested microenvironment supports axonal growth and maintains long-term structural stability, it may provide evidence that the substrate or scaffold design merits further investigation in neural tissue formation or repair. The data also help clarify how engineered surroundings provide adhesive cues to developing axons.