Fluorescent Bead Imaging
Fluorescent Bead Imaging - Related Videos
06:48
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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Cited by 3 •
2016
The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
06:42
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Cited by 32 •
2013
We review our recent results on the integration of fluorescent microscopy and imaging flow cytometry tools on a cell-phone using compact and cost-effective opto-fluidic attachments. These cell-phone based micro-analysis devices might be useful for cytometric analysis, such as performing various cell counting tasks as well as for high-throughput screening of e.g., water samples in resource limited settings.
11:41
Rapid Analysis and Exploration of Fluorescence Microscopy Images
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Cited by 3 •
2014
Here we describe a workflow for rapidly analyzing and exploring collections of fluorescence microscopy images using PhenoRipper, a recently developed image-analysis platform.
04:22
Live Imaging of the Drosophila Pupal Eye Using Fluorescence Microscopy
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2025
Source: Hellerman, M. B., et al. Live-imaging of the Drosophila Pupal Eye. J. Vis. Exp. (2015)The video demonstrates a live-imaging technique to study eye patterning in a Drosophila melanogaster pupa. Green fluorescent protein (GFP)-tagged markers enable visualization of cellular organization and differentiation during the formation of the developing eye structure.
FAQs
The key optical event is wavelength conversion: illumination at an appropriate excitation wavelength causes each bead to emit light at a longer wavelength. Fluorescence microscopy separates this emitted signal from the illumination and records bead positions or movement. Because the signal can be captured over time, researchers can convert otherwise difficult-to-observe dynamics into quantitative measurements.
Surface functionalization gives beads a way to target particular molecules or materials rather than serving only as passive optical markers. This added specificity can connect bead location or movement to a selected biological or engineered feature. In bioengineering studies, that relationship helps examine biomolecular interactions and material properties through measurable fluorescence patterns.
Recorded bead positions and trajectories can indicate how particles move through or respond to a biological or engineered environment. Interpreted measurements may describe fluid flow, particle transport, cellular forces, or interactions with tissues and scaffolds. The resulting optical data support quantitative analysis instead of relying only on qualitative visual observations.
A typical workflow places fluorescent beads within or against the biological or engineered system of interest, adds surface functionalization when molecular or material targeting is needed, and illuminates the preparation at a suitable excitation wavelength. Fluorescence microscopy then records emitted light and bead movement, producing data for measuring the selected process or structure.
In microfluidic systems, bead movement provides an optical means to examine fluid flow and particle transport within engineered environments. Imaging the emitted signal over time allows researchers to follow how beads move through the system and quantify transport-related behavior. These measurements can inform evaluation and design of microfluidic devices.
Bioengineering researchers can use bead-based measurements to investigate cellular forces, biomolecular interactions, and properties of tissues or scaffolds. The approach links observable bead signals with cell-environment interactions or engineered material behavior, helping researchers evaluate how systems function. Those measurements can contribute to biomaterial design and the assessment of tissue or scaffold performance.