Msc Culture
Msc Culture - Related Videos
11:05
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
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Cited by 21 •
2017
The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.
11:13
MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
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Cited by 47 •
2017
Here, a protocol (MitoCeption) is presented to transfer mitochondria, isolated from human mesenchymal stem cells (MSC), to glioblastoma stem cells (GSC), with the goal of studying their biological effects on GSC metabolism and functions. A similar protocol can be adapted to transfer mitochondria between other cell types.
04:49
MSC Seeded Scaffold Preparation: A Technique to Prepare Scaffold Seeded with Mesenchymal Stem Cells for Surgical Implantation into Murine Model
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2025
This video describes the technique of seeding mesenchymal stem cells (MSCs) onto a biodegradable poly(lactic acid) (PLA) scaffold. The prepared scaffolds can be implanted into the surgical resection cavity of the brain from where they may migrate towards tumor foci. Further, these cells can be engineered to express therapeutic proteins to stimulate apoptosis in cancer cells.
05:06
Fluorescence-guided Tumor Resection and MSC-seeded Scaffold Implantation in Murine Model: A Surgical Procedure to Deliver Therapeutic Mesenchymal Stem Cells into Brain Tumor Resection Cavity in Mouse Model
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2025
This video presents the surgical procedure for image-guided tumor resection and implantation of a bio-scaffold seeded with genetically engineered mesenchymal stem cells or MSCs expressing tumor necrosis factor-related apoptosis-inducing ligand or TRAIL. These therapeutic MSCs have the potential in treatment of brain tumors.
09:19
Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model
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Cited by 1 •
2020
Stem cell-based therapy has emerged as an efficient strategy to repair injured cardiac tissues after myocardial infarction. We provide an optimal in vivo application for stem cell transplantation using gelatin hydrogels that are able to be enzymatically cross-linked.
FAQs
These environmental conditions support cell survival, adhesion, and proliferation while helping maintain the characteristic properties of mesenchymal stem cells. Controlled temperature, pH, and gas composition create a reproducible setting for population growth. If conditions are not regulated, changes in cell behavior or properties may complicate comparisons between experiments and reduce the consistency of bioengineering studies.
Adhesion to a tissue-culture surface provides the physical foundation for maintaining and expanding MSC populations under laboratory conditions. Together with nutrient media and regulated environmental conditions, surface attachment supports continued cell proliferation. This relationship is especially relevant when researchers evaluate biomaterials, because the interaction between cells and engineered surfaces can influence how the culture performs.
Researchers use culture conditions to investigate and direct MSC differentiation toward bone, cartilage, or fat lineages. This makes the cells useful for studying how a multipotent population responds to bioengineering strategies. The resulting lineage-specific behavior can help evaluate approaches for tissue formation and compare how different materials or culture environments influence cell outcomes.
Standardized MSC culture methods help produce reproducible cell populations with more consistent growth and characteristic properties. Consistency matters when comparing experiments, assessing biomaterials, or examining directed differentiation. Without standardized conditions, variation in the laboratory environment or handling may make it difficult to determine whether observed outcomes arise from the tested bioengineering strategy or from the culture process itself.
A basic workflow begins by placing MSCs on tissue-culture surfaces and supplying nutrient media. Researchers then maintain regulated temperature, pH, and gas composition to support adhesion and proliferation as the population expands. The cultured cells can subsequently be used to study cell behavior, test biomaterials, or investigate differentiation toward selected tissue-related lineages.
Researchers use MSC culture when they need to examine how cells behave in the presence of biomaterials or within tissue-engineering strategies. Cultured MSCs provide a biological system for investigating adhesion, proliferation, and lineage-directed responses under controlled conditions. These observations can inform the assessment of materials intended for regenerative medicine and other bioengineering applications.
MSC culture can provide information about cell behavior, population expansion, and differentiation toward bone, cartilage, or fat lineages. It also supports evaluation of biomaterials and tissue-engineering strategies in a controlled laboratory setting. These outcomes contribute to broader research goals, including regenerative medicine, disease modeling, and translational studies that require reproducible cell populations.