Hl-60 Cells
Hl-60 Cells - Related Videos
08:25
Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer
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Cited by 9 •
2015
This article describes the technique used to perform dual channel optical mapping in cultured HL-1 atrial cell monolayers. This unique protocol allows the simultaneous visualization of both calcium (Ca) and voltage (Vm) activity in the same area for the detailed detection and analysis of electrophysiological properties of culture monolayers.
08:40
Preparation and Characterization of C60/Graphene Hybrid Nanostructures
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Cited by 1 •
2018
Here we present a protocol for the fabrication of C60/graphene hybrid nanostructures by physical thermal evaporation. Particularly, the proper manipulation of deposition and annealing conditions allow the control over the creation of 1D and quasi 1D C60 structures on rippled graphene.
11:04
Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
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Cited by 4 •
2013
This study used a multi-well plate microfluidic system, significantly increasing throughput of cell rolling studies under physiologically relevant shear flow. Given the importance of cell rolling in the multi-step cell homing cascade and the importance of cell homing following systemic delivery of exogenous populations of cells in patients, this system offers potential as a screening platform to improve cell-based therapy.
04:24
Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns
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Cited by 3 •
2011
We describe a protocol to observe and analyze cell rolling trajectories on asymmetric receptor-patterned substrates. The resulting data are useful for engineering of receptor-patterned substrates for label-free cell separation and analysis.
08:38
Electrospray Deposition of Uniform Thickness Ge23Sb7S70 and As40S60 Chalcogenide Glass Films
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Cited by 8 •
2016
A method of uniform thickness solution-derived chalcogenide glass film deposition is demonstrated using computer numerical controlled motion of a single-nozzle electrospray.
FAQs
Chemical cues such as dimethyl sulfoxide (DMSO) and all-trans retinoic acid (ATRA) can shift HL-60 cells toward a neutrophil-like phenotype, whereas other cues promote alternative myeloid states. This controllable response allows investigators to connect a defined treatment with differentiated cell behavior, supporting mechanistic studies of myeloid development and function.
Phenotypic control helps researchers examine how differentiation changes measurable functions rather than treating the cell population as uniform. Depending on the experimental cue, studies can evaluate migration, oxidative responses, or interactions with biomaterials and engineered tissue environments. Comparing these behaviors across induced states can reveal how cellular identity influences bioengineered systems.
Their rapid growth and experimentally controllable phenotype make HL-60 cells practical for in vitro experiments, but they do not fully replace primary human cells. Findings from differentiation, disease, drug-response, or immune-platform studies therefore require validation in primary human cells before broader biological conclusions are drawn.
A typical workflow begins by maintaining the cells under defined culture conditions, then exposing them to a selected chemical cue such as DMSO or ATRA. Researchers subsequently examine the resulting phenotype and relevant functions, including migration or oxidative responses. This sequence links the inducing condition to an experimentally observable myeloid state.
In bioengineering, HL-60 cells can serve as a controllable myeloid model for testing interactions with biomaterials or engineered tissue environments. Researchers can assess how a material or constructed setting relates to cell behavior, including migration and oxidative responses. The model supports early platform evaluation while retaining the need for validation in primary human cells.
Their rapid expansion and adjustable differentiation make HL-60 cells useful when experiments require a reproducible in vitro myeloid system. Investigators can apply defined conditions to study disease mechanisms, evaluate drug responses, or examine immune-cell-based platforms. The resulting observations provide an experimentally tractable starting point, although confirmation in primary human cells remains important.