In Vivo Collagen Remodeling
In Vivo Collagen Remodeling - Related Videos
07:03
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
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Cited by 18 •
2014
This procedure demonstrates in vivo near IR fluorescence imaging of collagen remodeling activities in mice as well as ex vivo staining of collagens in tissue sections using caged collagen mimetic peptides that can be photo-triggered to hybridize with denatured collagen strands.
02:54
Nucleosome Remodeling
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2020
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure. Nucleosome remodeling complex Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
10:24
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
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Cited by 16 •
2016
Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.
07:25
Collagen Hydrogels
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2023
Collagen is another widely used biomaterial that has found popularity in commercial applications, such as photography. Collagen has more recently been used in tissue engineering applications, by creating hydrogels that provide structure to engineered tissue. This video introduces collagen as a biomaterial, demonstrates how it is harvested from porcine skin, and shows how the material is used to create a hydrogel for tissue engineering applications. Finally, several applications of the material...
09:28
Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
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Cited by 9 •
2010
Creation of micro-tissues using cylindrical collagen gels, called modules, that contain embedded cells and which surface is coated with endothelial cells.
FAQs
Fibroblasts add and organize newly synthesized collagen, whereas matrix metalloproteinases and other proteases cleave existing fibers. The resulting balance between deposition and degradation allows tissue structure to change without losing mechanical integrity. In bioengineering, this coordination is important because a material or engineered tissue must accommodate both matrix replacement and host-driven restructuring.
Collagen architecture reflects more than the amount of material present. Cross-linking changes how collagen fibers are stabilized, while forces generated by cells and the surrounding tissue help organize their arrangement. Together with tissue mechanics, these factors shape matrix composition and structure, influencing whether remodeling supports adaptation, repair, or preservation of mechanical function.
Turnover indicates how actively existing collagen is being replaced, while alignment describes the organization of the resulting fibers. Examining both features can reveal how matrix structure changes during regeneration, fibrosis, or disease progression. These measurements therefore connect cellular remodeling activity with tissue-level architecture and help bioengineers evaluate whether a repair strategy restores an appropriate matrix organization.
Remodeling permits collagen-rich extracellular matrix to change as tissue requirements change. New matrix can be incorporated during growth or repair, while proteolytic removal of older material creates continued structural adjustment. Cross-linking, cellular forces, and tissue mechanics then help establish an architecture capable of maintaining integrity while accommodating the tissue’s changing functional demands.
Bioengineers use knowledge of host matrix restructuring to design biomaterials and engineered tissues that can integrate with living tissue. The remodeling process provides a framework for considering how deposited collagen, degraded matrix, cellular forces, and tissue mechanics will interact after implantation or incorporation, with the goal of supporting wound healing and restoring function.
Changes in collagen turnover, cross-linking, organization, and alignment can indicate how tissue structure is evolving over time. Comparing these features helps distinguish matrix changes associated with regeneration from patterns linked to fibrosis or disease progression. This context makes remodeling analysis useful for connecting extracellular-matrix architecture with broader tissue outcomes in bioengineering research.