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Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
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1Department of Cell and Regenerative Biology,University of Wisconsin-Madison, 2Laboratory for Optical and Computational Instrumentation,University of Wisconsin-Madison, 3Department of Biomedical Engineering,University of Wisconsin-Madison, 4Morgridge Institute for Research,University of Wisconsin-Madison, 5Paul P. Carbone Comprehensive Cancer center,University of Wisconsin-Madison
Chapters
- 00:05Title
- 01:00Polymerization of Collagen Solutions
- 02:23Generation of PDMS Microchannels for Collagen Fiber Alignment
- 04:34Preparation of the PDMS Microchannels and Collagen Pre-coating
- 06:41Pouring Collagen Microchannels
- 08:26Results: Cell Contractility and Fiber Alignment in Collagen Gels
- 09:26Conclusion
Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.
