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Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions <em>In Vivo</em>
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

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10:24 min

May 09, 2016

DOI:

10:24 min
May 09, 2016

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Summary

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Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.

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