Sheath Flow Device
Sheath Flow Device - Related Videos
08:58
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Cited by 8 •
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Microfluidic double emulsions generation typically involves devices with patterned wettability or custom-fabricated glass components. Here we describe the fabrication and testing of an all polydimethylsiloxane (PDMS) double emulsion generator that does not require surface treatment or complicated fabrication processes, and is capable of producing double emulsions down to 14 µm.
10:56
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The three-dimensional flow chamber device is a novel in vitro technology for the quantitative and step-wise evaluation of the extravasation cascade of cells circulating under conditions of physiological shear stress. The device therefore fills a critical need for basic, preclinical, and clinical studies of cell migration.
08:29
Thermal Measurement Techniques in Analytical Microfluidic Devices
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Cited by 6 •
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Here, we present three protocols for thermal measurements in microfluidic devices.
09:45
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Cited by 8 •
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Dielectrophoresis (DEP) is an effective method to manipulate cells. Printed circuit boards (PCB) can provide inexpensive, reusable and effective electrodes for contact-free cell manipulation within microfluidic devices. By combining PDMS-based microfluidic channels with coverslips on PCBs, we demonstrate bead and cell manipulation and separation within multichannel microfluidic devices.
07:28
Pitot-static Tube: A Device to Measure Air Flow Speed
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2023
Source: David Guo, College of Engineering, Technology, and Aeronautics (CETA), Southern New Hampshire University (SNHU), Manchester, New Hampshire A Pitot-static tube is widely used for measuring unknown speeds in air flow, for example, it is used to measure airplane airspeed. By Bernoulli's principle, airspeed is directly related to variations in pressure. Therefore, the Pitot-static tube senses the stagnation pressure and static pressure. It is connected to a manometer or pressure transducer...
FAQs
Under laminar-flow conditions, adjacent fluid layers move in an orderly manner, allowing the sheath fluid to narrow and center the sample stream without turbulent mixing. This controlled displacement produces a predictable flow profile across the device. As a result, particles or cells remain positioned consistently as they pass through the analysis region.
Consistent particle alignment places cells or particles in a repeatable position for optical interrogation. That stability helps measurements reflect differences among the analyzed objects rather than random changes in their location within the flow. In bioengineering systems such as flow cytometers, improved positioning supports more reproducible detection and characterization.
Sheath flow relies on orderly laminar motion to position the sample while preserving a controlled interface between the sample and surrounding fluid. Turbulent mixing would disrupt that predictable arrangement and make particle locations less consistent. The distinction matters because stable positioning improves measurement reproducibility and supports controlled manipulation in microfluidic devices.
Conceptually, the sample stream enters a region surrounded by sheath fluid, and the combined flow is maintained under laminar conditions. The sheath narrows and centers the sample before particles or cells reach the interrogation or manipulation region. Maintaining this controlled flow profile is central to obtaining consistent alignment and usable measurements.
Researchers may choose this approach when an experiment requires stable handling of cells or particles during counting, characterization, or optical analysis. It is especially relevant to flow cytometry, microfluidic diagnostics, and lab-on-a-chip systems. The device supports these uses by making the sample position more consistent and enabling reliable analysis at potentially high throughput.
A controlled sheath flow can improve the consistency of optical interrogation, cell counting, and particle characterization by presenting objects in a stable, repeatable position. The same fluid-handling principle also provides a foundation for cell sorting and other lab-on-a-chip technologies. Its value lies in connecting precise flow control with reproducible analytical or manipulative outcomes.