Blood Removal
Blood Removal - Related Videos
06:43
Rotary Evaporation to Remove Solvent
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2023
Source: Dr. Melanie Pribisko Yen and Grace Tang — California Institute of Technology Rotary evaporation is a technique most commonly used in organic chemistry to remove a solvent from a higher-boiling point compound of interest. The rotary evaporator, or "rotovap", was invented in 1950 by the chemist Lyman C. Craig. The primary use of a rotovap is to dry and purify samples for downstream applications. Its speed and ability to handle large volumes of solvent make rotary evaporation a preferred...
03:02
Nictitating Membrane Removal: A Surgical Procedure to Remove Nictitating Membrane from Rabbit Eye
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2025
In this video, we demonstrate a surgical procedure to remove nictitating membrane from rabbit’s eye.
03:56
Inferior Lacrimal Gland Removal From Rabbit Model: A Surgical Procedure to Remove the Larger Inferior Tear Gland From Rabbit Eye Orbit
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2025
In this video, we describe a surgical procedure to remove the large inferior lacrimal gland from a rabbit model to study the effect on tear production in the animal.
06:32
Blood Withdrawal II
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN The collection of blood from mice and rats for analysis can be done through a variety of methods. Each method of collection has variations in the type of restraint required, the invasiveness of the procedure, and the necessity of a general anesthetic.1Historically, the use of the retro-orbital sinus cavity has been used, but not without debate. The controversy related to the potential tissue...
06:17
Repeated Blood Collection for Blood Tests in Adult Zebrafish
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Cited by 87 •
2015
Repeated blood sampling is necessary and important in animal research. We developed a novel, non-lethal and reliable method for repeated blood collection from adult zebrafish, and applied this method to the study of blood biochemistry, including glucose metabolism.
FAQs
The amount withdrawn determines how much circulating volume and blood material are lost, while the withdrawal rate affects how quickly those changes occur. A larger or faster removal creates a greater immediate challenge to circulation than a smaller or slower one. Outcomes also depend on the organism’s capacity to restore fluid balance and replenish blood-cell populations.
The material affected depends on the purpose and design of the withdrawal. Blood removal may reduce circulating volume while removing whole blood, or it may target plasma, red cells, or other components for a specific biological or medical objective. This distinction matters because each component contributes differently to blood composition, hematology, and circulation.
Fluid balance influences how well an organism accommodates the reduction in circulating blood volume. Following withdrawal, recovery depends partly on the ability to restore fluids and reestablish stable circulation. The same removed volume may therefore produce different outcomes among organisms or experimental conditions, making fluid restoration an important variable when interpreting biological responses.
In biology, controlled withdrawal provides a way to examine changes in blood-related variables and circulatory function. Researchers can relate observed outcomes to the amount removed, the withdrawal rate, and the recovery of fluid balance or blood-cell populations. This makes the intervention useful for studying how organisms respond when circulating blood or specific components are reduced.
Blood removal begins by accessing a vessel or using a controlled incision, followed by allowing blood to leave through an appropriate route. A needle or catheter can provide vessel access, while an incision represents a different controlled approach. The selected method must match the organism, the intended sample or intervention, and the need to control withdrawal.
Diagnostic use obtains blood for analysis, allowing investigators or clinicians to examine the sample rather than primarily altering circulation. Blood donation collects blood for later use, whereas therapeutic phlebotomy deliberately withdraws blood as an intervention. These purposes differ in their goals, even though each depends on controlled access and withdrawal.
A study can evaluate blood composition, circulating volume, hematology, or circulatory responses, depending on what is removed and how the withdrawal is performed. Comparing outcomes across different amounts or rates can reveal how strongly the organism responds and how effectively it restores fluid balance or blood-cell populations over time.