Contact Mode Afm
Contact Mode Afm - Related Videos
08:21
Quantitative Hardness Measurement by Instrumented AFM-indentation
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Cited by 6 •
2016
This experimental protocol describes how atomic force microscopy can be used to measure hardness at the true nanometer scale and to detect single atomistic plasticity events.
09:47
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
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Cited by 3 •
2017
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
08:29
Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies
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Cited by 12 •
2016
To obtain basic information on the sorption and recycling of gold from aqueous systems the interaction of Au(III) and Au(0) nanoparticles on S-layer proteins were investigated. The sorption of protein polymers was investigated by ICP-MS and that of proteinaceous monolayers by QCM-D. Subsequent AFM enables the imaging of the nanostructures.
01:07
What is a Mode?
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2023
The mode is one of the commonly used measures of a central tendency. It is defined as the most frequent value in a data set. There can be more than one mode in a data set if multiple values have the same highest frequency. For instance, suppose that the Statistics exam scores of 20 students are: 50; 53; 59; 59; 63; 63; 72; 72; 72; 72; 72; 76; 78; 81; 83; 84; 84; 84; 90; 93. Here, the mode is 72, as it occurs most frequently, five times. A data set with two modes is called bimodal. For example,...
05:34
Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis
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Cited by 3 •
2023
Here, we present the application of atomic force microscopy (AFM) as a simple and fast method for bacterial characterization and analyze details such as the bacterial size and shape, bacterial culture biofilms, and the activity of nanoparticles as bactericides.
FAQs
As the sharp probe scans, forces between its tip and the sample bend the cantilever. The instrument detects this deflection and compares it with a defined force. A feedback system then adjusts the probe height to maintain that force, while the required height changes provide information about nanoscale surface topography.
The defined force establishes a consistent interaction between the probe and the specimen during scanning. Maintaining that condition allows cantilever deflection to be interpreted alongside probe-height changes, helping researchers characterize surface organization and structural features across biological samples rather than relying on a single local measurement.
The probe responds to interactions at the sample surface, and those responses are recorded during scanning. Consequently, the resulting measurements can describe nanoscale topography while also revealing mechanical features and differences in surface organization. This combination is useful for studying both the physical arrangement and structural properties of biological specimens.
A sharp probe is positioned against the sample so that it remains in continuous contact during scanning. As the probe moves across the surface, the system monitors cantilever deflection, applies feedback to maintain the selected force, and records the corresponding probe-height adjustments. These recorded changes are used to characterize the scanned surface.
The method can be applied to cell membranes, biomolecular assemblies, and other biological specimens that may be soft or rigid. This range makes it suitable for investigating nanoscale surface organization across different types of biological material, rather than restricting analysis to one specimen class or one physical state.
Measurements can provide information about surface organization, mechanical features, and structural changes. In biology, those outcomes support research in cell biology, microbiology, and biomaterials. Researchers can therefore use the technique to examine how biological surfaces and assemblies are arranged or how their observable structure differs across specimens.