Nitric Oxide Detection
Nitric Oxide Detection - Related Videos
08:58
En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
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Cited by 10 •
2016
In this article we describe an adapted relatively easy method using the fluorescence dye diaminofluorescein-2 diacetate (DAF-2DA) and dihydroethidium (DHE) for en face simultaneous detection and visualization of intracellular nitric oxide (NO) and superoxide anion (O2.−) respectively, in freshly isolated intact aortas of an obesity mouse model.
11:28
Analytical Techniques for Assaying Nitric Oxide Bioactivity
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Cited by 10 •
2012
The endogenous production of nitric oxide (NO) regulates a wide variety of biological functions. It is becoming increasingly clear that disruption or dysregulation of NO based signaling is involved in many human diseases. Methods to quantify relevant NO metabolites may provide novel diagnostic or prognostic biomarkers for human disease.
13:21
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
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Cited by 40 •
2012
Electron paramagnetic resonance (EPR) spectroscopy was employed to detect nitric oxide from bovine aortic endothelial cells and superoxide radical anion from human neutrophils using iron (II)-N-methyl-D-glucamine dithiocarbamate, Fe(MGD)2 and 5,5-dimethyl-1-pyroroline-N-oxide, DMPO, respectively.
07:49
Novel Whole-tissue Quantitative Assay of Nitric Oxide Levels in Drosophila Neuroinflammatory Response
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Cited by 24 •
2013
Levels of the inflammatory cell-signaling molecule nitric oxide (NO) are commonly assayed using Griess reagent. In this protocol, we have created a modified Griess assay utilizing live Drosophila brain tissue in order to detect the secretion of NO in a simple, quantifiable and highly repeatable method.
05:15
Inducing White Matter Stroke in a Murine Model Using a Nitric Oxide Inhibitor
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2025
This video demonstrates the precise technique for inducing a white matter stroke in an anesthetized mouse by injecting a nitric oxide inhibitor at targeted brain coordinates within the white matter. This leads to blood vessel constriction and inhibition of blood supply, resulting in neuronal damage and a brain stroke.
FAQs
A central strategy relies on NO’s rapid reactions with oxygen, superoxide, or heme centers. These reactions convert a short-lived signaling event into a measurable chemical or molecular change. The choice of reaction matters because it determines whether an experiment emphasizes direct reactivity, interaction with cellular heme-containing components, or formation of downstream products.
Fluorescent probes respond when NO-derived products modify a reporter molecule, producing a measurable fluorescence change. This approach can help reveal where NO-related activity occurs within cells or tissues, supporting spatial mapping of signaling. Interpretation depends on connecting the reporter response to NO-derived chemistry rather than treating fluorescence as an unqualified measure of NO itself.
Electrochemical approaches measure NO through its oxidation, whereas analytical assays quantify the more stable products nitrite and nitrate. The distinction separates measurements of NO-related electrochemical activity from measurements of accumulated products. Consequently, the two approaches can provide different views of the same biological system and may answer different questions about NO production.
These components influence the reactions that detection methods exploit. Oxygen and superoxide participate in NO chemistry, while heme centers provide another reactive context. Because NO is short-lived and rapidly transformed through such interactions, the surrounding biological chemistry affects which signal appears and how researchers interpret NO production in cells or tissues.
Researchers can match the method to the desired outcome: fluorescent reporters support cellular or tissue mapping, electrochemical oxidation measures NO-related electrochemical activity, and analytical assays quantify nitrite or nitrate. This selection links the measurement to the biological question, whether the goal is to examine signaling location, production changes, or stable chemical products.
In biology, these measurements can clarify how NO participates in signaling pathways and how its production changes across physiological or pathological conditions. Applications include examining inflammation, cardiovascular dysfunction, neuronal activity, and responses to therapies. Comparing signals across cells or tissues can connect NO-related chemistry with changes in vascular, neural, or immune activity.