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En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
 

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Article DOI: 10.3791/53718 08:58 min
February 25th, 2016

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Summary February 25th, 2016

In this article we describe an adapted relatively easy method using the fluorescence dye diaminofluorescein-2 diacetate (DAF-2DA) and dihydroethidium (DHE) for en face simultaneous detection and visualization of intracellular nitric oxide (NO) and superoxide anion (O2.−) respectively, in freshly isolated intact aortas of an obesity mouse model.

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