Stromal Cell-derived Inducing Activity
Stromal Cell-derived Inducing Activity - Related Videos
11:31
Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
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Cited by 21 •
2012
This protocol describes the isolation of adipose-derived stromal cells from lipoaspirate and the creation of a 4 mm critical-sized calvarial defect to evaluate skeletal regeneration.
12:40
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
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Cited by 91 •
2016
Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.
12:58
Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line
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Cited by 2 •
2017
Described here is a method for producing exogenous cytokine in patient-derived xenograft (PDX) mice via weekly intraperitoneal injection of a cytokine-transduced stromal cell line. This method broadens the utility of PDX and provides the option for transient or sustained exogenous cytokine delivery in a multitude of PDX models.
16:04
Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
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Cited by 66 •
2009
This protocol describes the isolation and subsequent expansion of mesenchymal stromal cells and endothelial colony forming cells without the use of animal serum to generate autologous pairs for experimental transplantation purposes.
09:07
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
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Cited by 1 •
2017
Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.
FAQs
The effect can arise through two linked routes: stromal cells signal while touching pluripotent stem cells, and they also release factors into the shared culture environment. Considering both routes is important because neural differentiation may not depend on a single isolated cue. The co-culture therefore provides a way to examine how contact-dependent and soluble influences jointly redirect cell fate.
It is considered instructive because exposure shifts pluripotent stem cells toward defined neural outcomes, including neural progenitor and neuronal lineages. This shift allows investigators to ask which environmental signals influence lineage commitment, rather than viewing the stromal cells only as a background culture component. The distinction is central to studying how surrounding cells regulate developmental decisions.
Multiple interacting components can make the inducing activity a systems-level phenomenon rather than the effect of one demonstrated signal. Studying the activity in this form lets researchers investigate combinations of cellular contact and released factors and consider how their combined influence produces neural differentiation. This perspective is useful when mapping the signals that control early lineage decisions.
A shift in pluripotent stem cells toward neural progenitor or neuronal lineages provides evidence that the surrounding cellular environment is influencing fate. These outcomes give researchers distinct developmental results to examine when studying lineage commitment. Comparing the neural cell types produced can also help connect stromal-cell signals with the progression of neural differentiation.
An experimental model requires pluripotent stem cells together with stromal cells in a shared co-culture context. The design should allow both direct cell contact and exposure to stromal-cell-released factors, because the activity may involve both forms of signaling. Researchers can then examine whether the stem-cell population shifts toward neural progenitor or neuronal lineages.
Researchers use the system when they need to investigate how cellular environments regulate neural fate, including questions about early nervous-system development and the signals controlling lineage commitment. The neural cells generated in the model can also support disease-modeling studies and regenerative research, linking developmental mechanisms with potential research applications.
The system provides a biological context for studying cell-based induction mechanisms, in which one cell population influences the developmental behavior of another. In this case, stromal-cell contact and released factors are examined for their effects on pluripotent stem-cell fate. That framework helps connect cellular communication with tissue development, neural differentiation, and experimental cell generation.